Fingerprinting temperate <Emphasis Type="Italic">japonica</Emphasis> and tropical <Emphasis Type="Italic">indica</Emphasis> rice genotypes by comparative analysis of DNA markers |
| |
Authors: | J?U?Jeung H?G?Hwang H?P?Moon Email author" target="_blank">K?K?JenaEmail author |
| |
Institution: | (1) Plant Breeding, Genetics, and Biotechnology Division, International Rice Research Institute, 7777, Metro Manila, Philippines;(2) Genetics and Breeding Division, National Institute of Crop Science, RDA, 209 Seodun-Dong, Suwon, 441-857, Republic of Korea;(3) IRRI-Korea Office, National Institute of Crop Science, RDA, 209 Seodun-Dong, Suwon, 441-857, Republic of Korea |
| |
Abstract: | This paper describes the relative efficiency of three marker systems, RAPD, ISSR, and AFLP, in terms of fingerprinting 14
rice genotypes consisting of seven temperatejaponica rice cultivars, three indica near-isogenic lines, three indica introgression lines, and one breeding line of japonica type adapted to high-altitude areas of the tropics with cold tolerance genes. Fourteen RAPD, 21 ISSR, and 8 AFLP primers
could produce 970 loci, with the highest average number of loci (92.5) generated by AFLP. Although polymorphic bands in the
genotypes were detected by all marker assays, the AFLP assay discriminated the genotypes effectively with a robust discriminating
power (0.99), followed by ISSR (0.76) and RAPD (0.61). While significant polymorphism was detected among the genotypes of
japonica and indica through analysis of molecular variance (AMOVA), relatively low polymorphism was detected within the genotypes of japonica rice cultivars. The correlation coefficients of similarity were significant for the three marker systems used, but only the
AFLP assay effectively differentiated all tested rice lines. Fingerprinting of backcross-derived resistant progenies using
ISSR and AFLP markers easily detected progenies having a maximum rate of recovery for the recurrent parent genome and suggested
that our fingerprinting approach adopting the ‘undefined-element-amplifying’ DNA marker system is suitable for incorporating
useful alleles from the indica donor genome into the genome of temperate japonica rice cultivars with the least impact of deleterious linkage drag. |
| |
Keywords: | rice japonica indica DNA fingerprinting DNA markers AMOVA |
本文献已被 SpringerLink 等数据库收录! |
|