| 麦二叉蚜传播玉米矮花叶病毒的机制 |
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| 引用本文： | 李向东,范在丰,李怀方,裘维蕃.麦二叉蚜传播玉米矮花叶病毒的机制[J].植物保护学报,2002,29(1):62-66. |
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| 作者姓名： | 李向东 范在丰 李怀方 裘维蕃 |
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| 作者单位： | 中国农业大学植物病理系,北京,100094 |
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| 基金项目： | 国家科委攀登计划资助项目(85-31-02-03) |
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| 摘 要： | 用胶体金标记法和荧光抗体标记法研究了麦二叉蚜(Schizaphis graminum)传播玉米矮花叶病毒(Maize dwaft mosaic virus,MDMV)的机制。麦二叉蚜传播玉米矮花叶病毒需要辅助成份-蛋白酶(Helper component-proteinase,HC-Pro)的参与。在电镜下观察到HC-Pro可以与MDMV粒子结合。用FITC标记的HC-Pro抗体和MDMV抗体证明,HC-Pro可以直接结合到蚜虫口针上;而MDMV粒子不能直接结合到蚜虫口针,必须在HC-Pro的辅助下才能结合到蚜虫口针上。这为HC-Pro在蚜虫传毒过程中起桥梁作用提供了新的证据。MDMV粒子主要吸附在蚜虫口针的尖端和中间部分。
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| 关 键 词： | 昆虫介体 植物病毒 玉米矮花叶病毒 辅助成份-蛋白酶 蚜虫 |
Mechanism of Schizaphis graminum in transmitting maize dwarf mosaic virus |
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| Li Xiangdong,Fan Zaifeng,Li Huaifang and Qiu Weifan.Mechanism of Schizaphis graminum in transmitting maize dwarf mosaic virus[J].Acta Phytophylacica Sinica,2002,29(1):62-66. |
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| Authors: | Li Xiangdong Fan Zaifeng Li Huaifang and Qiu Weifan |
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| Institution: | Department of Plant Pathology, China Agricultural University, Beijing 100094,Department of Plant Pathology, China Agricultural University, Beijing 100094,Department of Plant Pathology, China Agricultural University, Beijing 100094 and Department of Plant Pathology, China Agricultural University, Beijing 100094 |
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| Abstract: | Mechanism of Schizaphis graminum in transmitting maize dwarf mosaic virus studied by using immunogold labels and FTTC-labeled antibody. Helper component-proleinase ( HC-Pro) was necessary for S. graminum, to transmit maize dwarf mosaic virus (MDMV) . The binding of HC-Pro and MDMV particles was observed with immunogold under electronic microscopy. FTTC labeled antibody showed that HC-Pro directly bound with the stylets of S. graminum, MDMV particles only could bind the stylets of S. graminum with the help of HC-Pro. The distal and central parts of the stylets of S. graminum were the main areas where MDMV and HC-Pro were attached. These results provided further evidence for the bridge role of HC-Pro in transmitting poryviruses. |
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| Keywords: | insect vectors plant virus maize dwarf mosaic virus helper component-proteinase aphids |
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