| 海岛棉愈伤组织诱导及分化的影响因素初探 |
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| 引用本文： | 贺雅婷,曲延英,孔庆平,严勇亮,尚爽,郑良玉,陈全家.海岛棉愈伤组织诱导及分化的影响因素初探[J].分子植物育种,2008,6(3):597-602. |
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| 作者姓名： | 贺雅婷 曲延英 孔庆平 严勇亮 尚爽 郑良玉 陈全家 |
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| 作者单位： | 1. 新疆农业大学农学院,乌鲁木齐,830052
2. 新疆农业科学院经济作物研究所,乌鲁木齐,830000 |
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| 基金项目： | 国家自然科学基金
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新疆科技厅资助项目 |
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| 摘 要： | 本研究以新疆自育海岛棉品种新海16无菌苗下胚轴为外植体材料,进行愈伤组织诱导、分化及再生植株的初步研究,筛选出适合于海岛棉体细胞胚胎再生的培养体系。结果表明,最佳诱导愈伤组织及分化配方为MSB附加0.1mg/L2,4-D及0.05mg/LKT,分化率达到88%;于MSB1附加0.01mg/LKT及0.3mg/LNAA中对胚性愈伤组织进行增值培养;由MSB2附加0.1mg/LIBA中可分化出成熟体细胞胚胎及再生植株,分化率达73%。本研究通过体细胞胚胎发生途径获得了海岛棉再生植株,为海岛棉遗传转化体系的建立及分子育种奠定了基础。
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| 关 键 词： | 海岛棉 愈伤组织 分化 再生植株 下胚轴 |
Primary Studies on the Effects of Callus Induction and Differentiation in Gossypium Barbadense |
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| He Yating,Qu Yanying,Kong Qingping,Yan Yongliang,Shang Shuang,Zheng Liangyu,Chen Quanjia.Primary Studies on the Effects of Callus Induction and Differentiation in Gossypium Barbadense[J].Molecular Plant Breeding,2008,6(3):597-602. |
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| Authors: | He Yating Qu Yanying Kong Qingping Yan Yongliang Shang Shuang Zheng Liangyu Chen Quanjia |
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| Institution: | He Yating 1 Qu Yanying 1 Kong Qingping 2 Yan Yongliang 1 Shang Shuang 1 Zheng Liangyu 1 Chen Quanjia 11 Agriculture College of Xinjiang Agriculture University,Urumqi,830052,2 Institute of Cash Crops Research,Xinjiang Academy of Agricultural Sciences,830000 |
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| Abstract: | Hypocotyl of Xinhai 16, a breed of Gossypium barbadense cultured in Xinjiang, were used as explant to make research on callus induction, differentiation and plant regeneration, in hope of figuring out a favorable culture system for the regeneration of somatic embryo. The result indicates that the best culture medium for callus induction and differentiation is MSB media supplemented with 0.1 mg/L 2,4-D and 0.05 mg/L KT, with differentiation rates reaching 88%. In the culture medium of MSB1 supplemented with ... |
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| Keywords: | Gossypium barbadense Callus Differentiation Plant regeneration Hypocotyl |
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