| 小菜蛾肌球蛋白重链部分cDNA序列克隆、表达概况及分析 |
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| 引用本文： | 龚亮,钟国华,任珍珍,胡美英. 小菜蛾肌球蛋白重链部分cDNA序列克隆、表达概况及分析[J]. 江西农业大学学报, 2010, 32(1) |
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| 作者姓名： | 龚亮 钟国华 任珍珍 胡美英 |
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| 作者单位： | 华南农业大学,农药与化学生物学教育部重点实验室,广东,广州,510642 |
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| 基金项目： | 国家自然科学基金项目(30671387、30770291);;教育部全国优秀博士学位论文作者专项基金项目(2004061) |
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| 摘 要： | 肌肉肌球蛋白重链在生物运动过程中具有至关重要的作用,但在昆虫中此类基因极少被报道。首次克隆和分析小菜蛾肌球蛋白重链部分cDNA序列,GenBank登录号为FJ462712,其长408个碱基,推测编码135个氨基酸。同源分析表明:此多肽序列与凤蝶、家蚕、果蝇的同源性分别为95%,93%,82%。半定量RT-PCR研究表明:小菜蛾MHC基因在不同的生长发育阶段的表达皆不相同,在老熟幼虫和成虫中的表达量显著高于低龄幼虫和蛹。为进一步鉴定小菜蛾肌球蛋白重链的生理特征提供基因序列信息。
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| 关 键 词： | 小菜蛾 肌球蛋白重链 cDNA克隆 序列分析 表达概况 |
Cloning, Expression Profile and Sequence Analysis of Partial cDNA Coding for Myosin Heavy Chain from the Diamondback Moth,Plutella xylostella |
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| GONG Liang,ZHONG Guo-hua,REN Zhen-zhen,HU Mei-ying. Cloning, Expression Profile and Sequence Analysis of Partial cDNA Coding for Myosin Heavy Chain from the Diamondback Moth,Plutella xylostella[J]. Acta Agriculturae Universitis Jiangxiensis, 2010, 32(1) |
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| Authors: | GONG Liang ZHONG Guo-hua REN Zhen-zhen HU Mei-ying |
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| Affiliation: | Key Laboratory of Pesticide and Chemical Biology/a>;Ministry of Education/a>;South China Agricultural University/a>;Guangzhou 510642/a>;China |
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| Abstract: | Myosin heavy chain plays a central role in a molecular motor in biological motility but little work has been reported on this gene in insects.In this study,Pxyl-MHC,a partial cDNA(GeneBank accession NO:FJ462712) encoding myosin heavy chain of diamondback moth,Plutella xylostella was cloned,sequenced and analyzed.The cDNA is 408 bp that encodes 135 putative amino acids.The amino acid sequence exhibited a significant similarity to myosin heavy chain of Papilio xuthus(95%),Bombyx mori(93%) and Drosophila melan... |
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| Keywords: | Plutella xylostella myosin heavy chain (MHC) cDNA clone sequence analysis expression profile |
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