| 马铃薯块茎青枯病菌潜伏侵染的酶联免疫学检测 |
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| 引用本文： | 谢丛华 柳俊. 马铃薯块茎青枯病菌潜伏侵染的酶联免疫学检测[J]. 中国马铃薯, 2000, 0(3): 131-134 |
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| 作者姓名： | 谢丛华 柳俊 |
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| 作者单位： | 华中农业大学,湖北武汉 |
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| 摘 要： | 试验采用武汉市场上来源不同产地的 7份马铃薯材料 ,用CIP提供的RS NCM ELISA试剂盒进行了块茎青枯病菌携带情况的检测。试验结果显示 ,应用该试剂检测马铃薯块茎青枯病菌携带情况具有较高的灵敏度 ;检测前样品的富集时间对检测结果具有明显的影响 ,其最佳富集时间为4 8h ;不同来源的马铃薯块茎携带青枯病菌的程度具有显著差异
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| 关 键 词： | 马铃薯块茎 青枯病 潜伏侵染 RS-NCM-ELISA |
DETECTION OF RALSTONIA SOLANACEARUM IN LATENTLY INFECTED POTATO TUBERS BY ENZYME LINKED IMMUNOSORBENT ASSAY |
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| XIE Cong hua,LIU Jun and ZHANG Ke shun. DETECTION OF RALSTONIA SOLANACEARUM IN LATENTLY INFECTED POTATO TUBERS BY ENZYME LINKED IMMUNOSORBENT ASSAY[J]. Chinese Potato, 2000, 0(3): 131-134 |
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| Authors: | XIE Cong hua LIU Jun ZHANG Ke shun |
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| Abstract: | Potato tubers purchased in Wuhan market from seven sources,were used for detection of Ralstonia solanacearum using RS NCM ELISA kit kindly provided by CIP(Interrnational Potato Center).The results showed that the kit was sensitive and applicable to potatoes.Variations in degree of infection among samples from different locations were identified,indicating a high risk of using these tubers as seeds.The time of bacteria enrichment before detecting affected the results remarkably.We confirmed that the approieet time for enrichment was 48 hr under the conditions of this expriment. |
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| Keywords: | potato ralstonia solanacearum latent infection NCM ELISA(Enzyme linked immunosorbent assay on nitrocellulose membrane) |
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