蔬菜及其制品中农药残留测定方法的探讨

建立蔬菜及其制品中的杀虫剂艾氏剂、狄氏剂、异狄氏剂残留的气相色谱检测方法。采用Agilent 6890N色谱仪,以高纯氮(纯度>99.999%)为载体,利用程序升温,用ECD检测器进行艾氏剂、狄氏剂、异狄氏剂定性定量分析。3种杀虫剂浓度在0.001~0.500 mg/kg时,回收率在90%~100%,相对标准偏差(RSD)<5%。艾氏剂、狄氏剂、异狄氏剂在0.001~0.500 mg/L浓度范围内有良好的线性。以3倍信噪比(S/N)为检出限,采用该方法对3种杀虫剂的检测限分别为:艾氏剂为0.3μg/kg;狄氏剂为0.5μg/kg;异狄氏剂为0.3μg/kg。该方法可同时测定艾氏剂、狄氏剂和异狄氏剂,并具有步骤简单,操作性强的特点,其测定结果具有较高的准确度和变异系数。

Discuss on Determining Methods of Pesticide Residue in Vegetable and its Products

The purpose was to set up the gas chromatography method of determining the pesticide residues of Aldrin,Dieldin and Endrin in vegetable and its products.With high-purity nitrogen(purity > 99.999%) as carrier,Agilent 6890N chromatograph,the programmed temperature method and ECD detector were used to do the qualitative and quantitative analyses on Aldrin,Dieldin and Endrin.When the 3 kinds of pesticides were in the concentration scope of 0.001～0.500 mg/kg,their recoveries were in 90%～100%,with relative standard deviation(RSD)smaller than 5%.Aldrin,Dieldin and Endrin had good linearity in their concentration scopes of 0.001～0.500 mg/L.3 times of signal-to-noise ratio(S/N)being taken as detection limit,the limits of 3 kinds of pesticides detected by this method were:Aldrin of 0.3 μg/kg,Dieldin of 0.5 μg/kg and Endrin of 0.3 μg/kg respectively.The method can determine Aldrin,Dieldin and Endrin at the same time,with characters of simple approaches and strong operability.Their determination results showed higher accuracy and variation coefficient.