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| 玉米SSR标记的多重PCR试验分析 | |
| 引用本文： | 王伟,杨文鹏,戴保威.玉米SSR标记的多重PCR试验分析[J].贵州农业科学,2009,37(12). |
| 作者姓名： | 王伟 杨文鹏 戴保威 |
| 作者单位： | 1. 贵州省旱粮研究所,贵州,贵阳,550006;贵州省农业生物技术重点实验室,贵州,贵阳550006;贵州大学,农学院,贵州,贵阳,550025 2. 贵州省旱粮研究所,贵州,贵阳,550006;贵州省农业生物技术重点实验室,贵州,贵阳550006 3. 贵州大学,农学院,贵州,贵阳,550025 |
| 基金项目： | 贵州省科技计划项目"贵州省农作物分子标记应用技术研究"，国家科技支撑计划项目"喀斯特山区特有作物优异基因资源发掘与转基因安全关键技术研究与应用" |
| 摘 要： | 以玉米自交系4011为试验材料,选用玉米SSR核心引物20对,利用单重SSR分子标记技术体系,采用逐步递加引物对的方法,尝试了二重、三重、四重、五重、六重PCR试验.结果筛选出了6个适宜六重PCR扩增的SSR标记组合:phi053/umc2084/umc1196/bnlg1154/mmc0151/bnlg1025、umc1231/umc1196/phi053/umc1222/mmc0151/bnlg1025、umc1231/umc1196/phi053/umc1222/bnlg240/bnlg1025、umc1231/umc1196/phi053/umc1222/1154/bnlg249、bnlg1451/umc1196/phi053/umc1019/phi072/bnlg1025、bnlg1451/umc1196/phi053/umc1545/bnlg2291/bnlg1025.这些标记组合的PCR扩增和PAGE电泳检测良好,从而可提高SSR标记检测的效率,并且降低试验成本. |
| 关 键 词： | 玉米 SSR标记 多重PCR |
Multiple PCR Analysis of Maize SSR Markers |
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| WANG Wei,YANG Wen-peng,DAI Bao-wei.Multiple PCR Analysis of Maize SSR Markers[J].Guizhou Agricultural Sciences,2009,37(12). | |
| Authors: | WANG Wei YANG Wen-peng DAI Bao-wei |
| Abstract: | 4011, a maize inbred line, was tested by using 20 pairs of core maize SSR primers based on the single SSR marker technique to test the two, three, four, five and six fold PCR amplification technique. Six SSR marker combinations of phi053/umc2084/umc1196/bnlg1154/mmc0151/bnlg1025, umc1231/umc1196/phi053/umc1222/mmc0151/bnlg1025, umc1231/umc1196/phi053/umc1222/bnlg240/bnlg1025, umc1231/umc1196/phi053/umc1222/1154/bnlg249, bnlg1451/umc1196/phi053/umc1019/phi072/bnlg1025 and bnlg1451/umc1196/phi053/umc1545/bnlg2291/bnlg1025 to be suitable to six-fold PCR amplification were selected.These marker combinations with good PCR amplification and PAGE lectrophoretic examination can improve the examination efficiency of SSR marker nd reduce the test cost effectively. |
| Keywords: | maize SSR marker multiple PCR |
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