| EGCG3" Me提取与分离工艺研究 |
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| 引用本文： | 罗正飞,金孝芳,龚正礼,梁名志,汪毅,王立波,陈继伟.EGCG3" Me提取与分离工艺研究[J].茶叶科学,2011,31(3):218-224. |
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| 作者姓名： | 罗正飞 金孝芳 龚正礼 梁名志 汪毅 王立波 陈继伟 |
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| 作者单位： | 云南省农业科学院茶叶研究所;西南大学食品科学学院;湖北省农业科学院果树茶叶研究所;重庆长城茶叶贸易有限公司; |
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| 基金项目： | 重庆市科委项目资助(CSTC.2007AC1044) |
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| 摘 要： | 采用HPLC、聚酰胺树脂、HPTLC等技术手段,进行EGCG3"Me的提取与分离纯化研究.通过正交实验表明,EGCG3"Me最优提取工艺条件为:乙醇体积分数70%、回流时间3 0 min、回流温度80℃、料液比1:10,质量分数为0.89%.采用40%酒精、60%酒精进行冼脱时,有大量EGCG3"Me富集.以甲醇:氯仿...
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| 关 键 词： | EGCG3"Me 提取 分离 HPLC 聚酰胺 |
Study on Extraction and Separation Process of EGCG3Me |
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| LUO Zheng-fei,JIN Xiao-fang,GONG Zheng-li,LIANG Min-zhi,WANG Yi,WANG Li-bo,CHEN Ji-wei.Study on Extraction and Separation Process of EGCG3Me[J].Journal of Tea Science,2011,31(3):218-224. |
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| Authors: | LUO Zheng-fei JIN Xiao-fang GONG Zheng-li LIANG Min-zhi WANG Yi WANG Li-bo CHEN Ji-wei |
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| Institution: | LUO Zheng-fei1,2,JIN Xiao-fang2,3,GONG Zheng-li2,LIANG Min-zhi1,WANG Yi4,WANG Li-bo1,CHEN Ji-wei11.College of Food Science,Southwest University,Chongqing 400716,China,2.Tea Research Institute,Yunnan Academy of Agricultural Sciences,Menghai 666201,3.Fruit and Tea Research Institute,Hubei Academy of Agricultural Sciences,Wuhan 430209,4.Chongqing Great Wall Tea Trade Company,Chongqing 400020,Chin |
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| Abstract: | The extraction and separation process of EGCG3"Me was studied by HPLC,polyamide resin and HPTLC methods.According to the orthogonal experimental design,the optimum extracting conditions of EGCG3"Me were as follows: alcohol concentration 70%,reflux time 30 min,reflux temperature 80℃ and solid-liquid ratio 1:10.And the yield of EGCG3"Me was 0.89%.Large amount of EGCG3"Me was found in the elution of alcohol concentration 40% and 60%.With elution of methanol: chloroform(7:2),the high purity EGCG3"Me was obtained by further separation and purification of 100～120 mesh polyamide resin. |
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| Keywords: | EGCG3Me extraction separation HPLC polyamide |
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