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暗褐网柄牛肝菌菌株分离研究
引用本文:何明霞,王文兵,张春霞,刘静,曹旸,高锋,周茂,纪开萍.暗褐网柄牛肝菌菌株分离研究[J].中国食用菌,2013(6):12-14.
作者姓名:何明霞  王文兵  张春霞  刘静  曹旸  高锋  周茂  纪开萍
作者单位:云南省热带作物科学研究所,云南景洪666100
基金项目:国家自然科学基金资助项目“暗褐网柄牛肝菌营养方式及菌丝胞外酶活性研究”(31060271);农业科技成果转化资金项目“暗褐网柄牛肝菌仿生栽培发明专利技术成果转化与示范”(2011GB217300004);景洪市科技三项费项目“黑牛肝菌人工栽培技术转化与示范”(2013.46).
摘    要:通过对培养基、分离材料2个方面5个因素进行研究,结果表明,分离培养暗褐网柄牛肝菌菌株以采集的中熟子实体的菌肉分离效果最好,采后到分离时间不宜超过24h,培养基以M1配方为最好。

关 键 词:暗褐网柄牛肝菌  菌株分离  分离方法

Studies on Isolating of Phlebopus portentosus Strain
HE Ming-xia,WANG Wen-bing,ZHANG Chun-xia,LIU Jing,CAO Yang,GAO Feng,ZHOU Mao,JI Kai-ping.Studies on Isolating of Phlebopus portentosus Strain[J].Edible Fungi of China,2013(6):12-14.
Authors:HE Ming-xia  WANG Wen-bing  ZHANG Chun-xia  LIU Jing  CAO Yang  GAO Feng  ZHOU Mao  JI Kai-ping
Institution:(Yunnan Institute of Tropical Crops, Jinghong Yunnan 666100)
Abstract:Five factors concerning the culture medium, the isolating material were studied. It showed that the best materials were tubuli of the middle fructification that were collected. Time should not exceed 24 hours from collect to isolate. M1 was the best medium.
Keywords:Phlebopus portentosus  Strain isolating  The way of isolating
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