不同温度下BDE-209对草鱼离体肝脏组织的毒性效应

为探讨不同温度条件下十溴联苯醚(BDE-209)对鱼类的毒性影响,以草鱼(Ctenopharyngodon idellus)幼鱼离体肝脏组织为试验材料,在肝脏组织匀浆液中加入浓度为0.00、0.04、4.00、400.00μg/L的BDE-209,并将其分别置于低温(15℃)、常温(25℃)、高温(35℃)中水浴60 min,然后检测肝脏组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽还原酶(GR)的活性以及丙二醛(MDA)的含量。结果显示:低温时各浓度组BDE-209诱导下的SOD、CAT、GR活性与对照组没有显著性差异;常温时BDE-209对SOD、CAT、GR活性具有低浓度诱导高浓度抑制的现象,高温时BDE-209对肝脏组织中CAT、SOD、GR活性造成明显的抑制效应,MDA在不同温度条件下其含量都随BDE-209暴露浓度的升高而升高。研究结果表明:在一定的温度范围内,温度越高,BDE-209对鱼体肝脏组织造成的氧化损伤越大。

Toxic effects of BDE-209 on isolated liver tissues of Ctenopharyngodon idellus at different temperatures

In order to investigate the toxicity of decabromodiphenyl ether(BDE-209)to fish under different temperature conditions,the liver tissue of juvenile Ctenopharyngodon idellus were used in vitro study.The liver homogenate was added BDE-209 to the final concentration 0.00,0.04,4.00,400.00μg/L and incubated at 15℃,25℃,35℃for 60 min,respectively.The activities of superoxide dismutase(SOD),catalase(CAT),glutathione reductase(GR)and the content of malondialdehyde(MDA)in liver tissue were detected.The results showed that the activities of SOD,CAT and GR were not significant compared to the control group after exposure to BDE-209 at low temperature.The activities of SOD,CAT,GR were increased at low concentration and decreased at high concentration after exposure to BDE-209 at normal temperature.The activities of SOD,CAT and GR were significantly decreased after exposed to BDE-209 at high temperature.The MDA contents were increased along with BDE-209 doses at all temperature treated groups.The results showed that elevated temperature will enhance the oxidative damage of BDE-209 on liver tissue of fish within a certain temperature range.