FK506结合蛋白FKBP52对金龟子绿僵菌侵染东亚飞蝗的影响

为了明确东亚飞蝗Locusta migratoria manilensis免疫相关FK506结合蛋白(FK506 binding protein,FKBP)的功能,本研究采用荧光定量PCR分析了FKBP52基因在成虫不同组织以及不同龄期中肠中的表达量;克隆并表达了FKBP52基因,纯化获得目的蛋白;测定了FKBP52蛋白对金龟子绿僵菌IMI330189侵染东亚飞蝗及东亚飞蝗体内保护酶酶活性的影响。研究结果发现FKBP52基因在成虫不同组织中均有表达,中肠中相对表达量最高;在不同龄期的中肠中,以卵期和成虫期的相对表达量较高。FKBP52基因全长1242 bp,编码蛋白分子量为46 kDa。生测及酶活力测定结果表明,金龟子绿僵菌IMI330189与FKBP52混合处理时,东亚飞蝗第10天累计死亡率显著提高到93.33%,东亚飞蝗体内的保护酶活力均显著降低。上述结果表明,FKBP52基因能够通过抑制东亚飞蝗体内保护酶活性,促进金龟子绿僵菌IMI330189的侵染。

Effects of FK506-binding protein FKBP52 on Metarhizium anisopliae infesting Locusta migratoria manilensis

In order to clarify the function of FK506-binding protein(FKBP)of immunity in Locusta migratoria manilensis,the expression of FKBP52 gene in different tissues and midgut of different stages was analyzed by qPCR.The FKBP52 gene was cloned and expressed,and the target protein was purified.The effect of FKBP52 protein on the protective enzyme activity of L.migratoria manilensis infected by Metarhizium anisopliae IMI330189 and the mortality were described.The study found that the FKBP52 gene was expressed in different tissues of adults,and the relative expression was the highest in the midgut.In the midgut of different stages,the relative expression was higher at egg stage and adult stage.The full length of FKBP52 gene was 1242 bp,and the molecular weight of the encoded protein was 46 kDa.The results of bioassay and enzyme activity determination showed that,when M.anisopliae IMI330189 was mixed with FKBP52,the cumulative mortality of L.migratoria manilensis on the 10th day increased significantly to 93.33%,and the activity of protective enzymes decreased significantly.These results showed that FKBP52 gene could promote the infection of M.anisopliae IMI330189 by inhibiting the activity of protective enzymes in L.migratoria manilensis.