| 鹿茸干细胞体外培养技术的研究 |
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| 引用本文： | 孙红梅,邢秀梅,丛波,李春义,杨福合.鹿茸干细胞体外培养技术的研究[J].经济动物学报,2007,11(1):7-9,14. |
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| 作者姓名： | 孙红梅 邢秀梅 丛波 李春义 杨福合 |
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| 作者单位： | 中国农业科学院特产研究所,吉林,132109 |
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| 摘 要： | 利用消化酶消化法,用DMEM培养基对鹿茸干细胞进行了体外培养,分别从原代培养、传代、冻存、以及复苏等几个环节进行了试验。摸索鹿茸干细胞合适的体外培养条件,为鹿茸的各项相关研究打基础。结果表明,用透明质酸酶和胶原酶对组织样进行消化,然后用10%DMEM培养基进行培养细胞生长效果较好。适宜培养条件是37℃、5%CO2最大饱和湿度。
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| 关 键 词： | 鹿茸 干细胞 体外培养 |
| 文章编号： | 1007-7448（2007）01-0007-03 |
| 修稿时间： | 2006-07-14 |
Cultural Technique of Deer Antler Stem Cell in Vitro |
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| SUN Hong-mei,XING Xiu-mei,CONG Bo,LI Chun-yi,YANG Fu-he.Cultural Technique of Deer Antler Stem Cell in Vitro[J].Journal of Economic Animal,2007,11(1):7-9,14. |
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| Authors: | SUN Hong-mei XING Xiu-mei CONG Bo LI Chun-yi YANG Fu-he |
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| Institution: | The Institute of Special Economic Animal and Plant Sciences, CAAS, Jilin 132109, China |
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| Abstract: | The culture conditions of deer antler cell in vitro were studied in the research.The result showed that the cell could be cultured in vitro with the hyaluronidase and collagenase to digest tissue and grow well in the medium of 10% FCS DMEM.The optimized culture condition was in the environment of 37℃,5%CO2 and saturated humidity. |
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| Keywords: | deer antler stem cell culture in vitro |
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