| 应用蛋白质和DNA片段电泳分析鉴定稻种上的黄色细菌 |
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| 引用本文： | 叶华智 帅正彬. 应用蛋白质和DNA片段电泳分析鉴定稻种上的黄色细菌[J]. 四川农业大学学报, 1993, 11(3): 339-345 |
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| 作者姓名： | 叶华智 帅正彬 |
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| 作者单位： | 四川农业大学农学系，四川农业大学农学系，四川农业大学农学系 雅安市 625014，雅安市 625014，雅安市 625014 |
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| 摘 要： | 本研究的目的是建立一种能简便、快速、准确鉴定水稻种子上寄藏的三类黄色细菌的方法。通过对三类菌株的菌体蛋白质和DNA限制性片段的电泳分析结果表明,三类细菌的蛋白质和DNA限制性片段电泳图谱明显不同,而在同一类群内不同菌株间的电泳图型则基本相同,因此能应用蛋白质和DNA限制性片段电泳分析于这些类群细菌的分类鉴定和亲缘关系分析,特别是菌体蛋白质是电泳分析具有简便、快速等优点。
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| 关 键 词： | 细菌(45LA) 水稻(49E) 电泳(32KA34BC65CH) 凝胶电泳分析 黄包细菌 菌体蛋白质 DNA限制性片段 |
IDENTIFICATION OF YELLOW—PIGMENTED BAVTERIA HABRING IN RICE SEEDS BY ELECTROPHORESIS ANALYSIS OF CELLU—IAR PROTEIN AND DNA REST.RICTION FRAGMENT |
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| Ye Huazhi et al. IDENTIFICATION OF YELLOW—PIGMENTED BAVTERIA HABRING IN RICE SEEDS BY ELECTROPHORESIS ANALYSIS OF CELLU—IAR PROTEIN AND DNA REST.RICTION FRAGMENT[J]. Journal of Sichuan Agricultural University, 1993, 11(3): 339-345 |
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| Authors: | Ye Huazhi et al |
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| Abstract: | The objective of the present study was to construct a rapid simple and reliable method for the diffrentiation of yellow-pigmen-ted bacteria habring in rice seeds. The callula protein and DNA restriction fragment from yellow-pigmented bacteria belonging to three groups identfied by morphlogical, physiological and biological features, and pathogenic tests were subjected to poly-acrymide vetical of agrose horizontal gel electrophoresis for their prontein and DNA restriction fragment patternsm, The results showed that the patterns of prontein and DNA frahment from diffrer nt/groups were very distinct from each other, but the paterns of different strains in the same group were similar, so that the electro phoresis analysis can be used as a taxonomic criteria for diffe nting them and the relationgship among |
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| Keywords: | BACTERIA LOWLAND RICE ELECTROPHORESIS RICE BACTERIAL LEAF BLIGHT BACTERIAL LEAF STREAK PROTEIN DNA RESTRICTION FRAGMENT |
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