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| 北京鸭PRLR基因的PCR-SSCP检测 | |
| 引用本文： | 赵楠,刘小林,侯水生,王杰,黄苇. 北京鸭PRLR基因的PCR-SSCP检测[J]. 西北农业学报, 2008, 17(3): 42-45 |
| 作者姓名： | 赵楠 刘小林 侯水生 王杰 黄苇 |
| 作者单位： | 1. 西北农林科技大学动物科技学院,陕西杨凌,712100 2. 中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室,北京,100094 |
| 基金项目： | 国家科技支撑计划 , 西北农林科技大学博士基金 |
| 摘 要： | 为确定催乳素受体(PRLR)基因与北京鸭产蛋性能的关系,利用PCR-SSCP技术检测北京鸭2个品系(W2系、Z2系)238个个体PRLR基因第9内含子的单核苷酸多态性(SNP),结果未见SNP。测序后经序列比对发现与NCBI数据库中(GenBank登录号EF502053)序列一致。由于内含子上的突变不会引起编码蛋白质的变化,所以更适用于品种间的比较,可在今后的研究中进行遗传多样性研究。而外显子的突变更能对性状产生影响,可在今后加大研究力度。 |
| 关 键 词： | 北京鸭 催乳素受体(PRLR) PCR-SSCP |
| 收稿时间： | 2007-10-17 |
| 修稿时间： | 2007-12-30 |
PCR-SSCP Detection of Prolactin Receptor (PRLR) Gene in Peking Duck |
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| ZHAO Nan,LIU Xiao-lin,HOU Shui-sheng,WANG Jie and HUANG Wei. PCR-SSCP Detection of Prolactin Receptor (PRLR) Gene in Peking Duck[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2008, 17(3): 42-45 | |
| Authors: | ZHAO Nan LIU Xiao-lin HOU Shui-sheng WANG Jie HUANG Wei |
| Affiliation: | College of Animal Sci-tech, Northwest A & F University, Yangling Shannxi 712100, China;College of Animal Sci-tech, Northwest A & F University, Yangling Shannxi 712100, China;State Key Laboratory of Animal Nutrition Science, Institute of Animal Science, CAAS, Beijing 100094, China;College of Animal Sci-tech, Northwest A & F University, Yangling Shannxi 712100, China;State Key Laboratory of Animal Nutrition Science, Institute of Animal Science, CAAS, Beijing 100094, China |
| Abstract: | To make sure the correlation between PRLR gene and laying performance,PCR-SSCP was applied to detect single nucleotide polymorphism(SNP) of intron 9 of prolaction receptor(PRLR) gene for 238 ducks in Peking duck,including two lines(W2,Z2).The results demonstrated no polymorphism.After sequenced,the DNA sequence was conformity with the sequence in NCBI(GenBank Accession EF EF502053).The analysis of single nucleotide polymorphism in intron is more applicable among species;because the mutation in intron can't change encoding protein.So study on genetic diversity must be feasible.Due to the mutation in exon can affect the traits,more researh should be done. |
| Keywords: | Peking duck Prolactin receptor(PRLR) PCR-SSCP |
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