Repeated sequence RSα is diagnostic for Bradyhizobium japonicum and Bradyrhizobium elkanii |
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| Authors: | A Hartmann M Gomez J J Giraud C Revellin |
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| Institution: | (1) Centre de Microbiologie du Sol et de l‘Environnement, Laboratoire de Microbiologie des Sols, INRA Dijon, 17 rue Sully, BV 1540, F-21034 Dijon cedex, France, FR |
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| Abstract: | The genome of Bradyrhizobium japonicum and B. elkanii contains multiple copies of the repeated DNA sequence RSα. A collection of 18 B. japonicum, 4 B. elkanii and 72 other bacterial strains was screened by polymerase chain reaction (PCR) using a pair of primers specific for RSα.
Only strains of B. japonicum and B. elkanii gave the predicted amplification product. Restriction analysis of PCR products obtained from different strains of B. japonicum showed that the RSα sequence was generally conserved. The usefulness of RSα as a specific probe for Bradyrhizobium strains capable of nodulating soybean was also demonstrated.
Received: 11 May 1995 |
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| Keywords: | Bradyrhizobium japonicum Bradyrhizobium elkanii Repeated sequence RSα Polymerase chain reaction detection DNA hybridization Soil inoculant Nodulation tests N2 fixation |
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