不同类型小麦品种孕穗期低温生理反应及其抗寒性分析

为明确冬小麦抗低温倒春寒的生理机制,以多穗型小麦品种济麦22、邯6172和大穗型小麦品种临麦4号、潍麦8号为材料,研究了孕穗期低温(1℃、-2℃)对小麦叶片可溶性糖、可溶性蛋白、脯氨酸和丙二醛(MDA)含量以及超氧化物歧化酶(SOD)活性的影响。结果表明,在低温处理当天,1℃低温下,4个品种的可溶性糖、可溶性蛋白、脯氨酸和MDA含量均不同程度增加,SOD活性除济麦22外均提高;-2℃低温下,可溶性糖和脯氨酸含量增加更明显,SOD活性除临麦4号外均降低,各品种MDA含量均显著减少。低温处理第3d和第6d,低温最初诱导产生的脯氨酸逐渐减少,MDA含量大幅降低,其余指标均增加。经用隶属函数法综合分析,4个品种的抗寒性相近,穗型间差异不明显。

Analysis on the Physiological Reaction to Low Temperature at Booting Stage and Their Cold Resistance of Different Types of Wheat Varieties

Using multi-spike wheat varieties(Jimai22,Han6172) and large-spike wheat varieties(Linmai4,Weimai8) as experimental materials,the dynamic variation of soluble sugar,soluble protein,proline,SOD and MDA were investigated at different days(0,3,6) after different temperature(normal,1℃,-2℃) treatments.The results indicated that,compared to the control,the content of soluble sugar increased a little at 1℃,while significantly(P<0.05) increased at-2℃ in the leaves;the content of soluble protein significantly increased at 1℃ and-2℃,and the increasing amount under the two different temperature treatments was similar;the content of proline significantly increased at 1℃,while greatly increased at-2℃;the activity of SOD was varied with different varieties,some increased and some decreased;the content of MDA significantly increased at 1℃,but decreased dramatically at-2℃.At 3 days after treatment,all of the three indexes gradually increased except proline content and MDA content,and the difference of soluble sugar content and protein content between different temperatures gradually increased.At 6 days after treatment,a similar changing trend to that before was observed.At last,by using fuzzy mathematics method,the cold resistance ability of those winter wheat varieties was evaluated,it is found that the cold resistance of those cultivars were similar and there was no correlation between spike type and cold resistance.