| 一种植物表达载体的构建及其在金柑上的瞬时表达 |
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| 引用本文： | 张岚岚,刘小花,陈昆松,徐昌杰.一种植物表达载体的构建及其在金柑上的瞬时表达[J].果树学报,2008,25(3):431-434. |
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| 作者姓名： | 张岚岚 刘小花 陈昆松 徐昌杰 |
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| 作者单位： | 浙江大学华家池校区园艺系/农业部园艺植物生长发育与生物技术重点开放实验室,杭州,310029 |
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| 基金项目： | 国家自然科学基金
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浙江省自然科学基金 |
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| 摘 要： | 以植物表达载体pCambia1301的带内含子的GUS(iGUS)基因替换植物表达载体pBI121中的GUS基因,构建了以pBI121为基础载体并含有iGUS的植物表达载体pLZ13。农杆菌染色表明,pCamiba1301和pLZ13两载体中的iGUS基因均不会导致GUS染色反应。以金柑不同组织为材料,通过农杆菌介导开展瞬时表达研究,结果表明,pLZ13和pCambia1301两载体的iGUS在CaMV35S启动子调控下的表达没有差异,证明构建的pLZ13是一种同时适于瞬时表达和转基因研究的植物表达载体。
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| 关 键 词： | 金柑 iGUS 瞬时表达 启动子 转基因 植物表达载体 |
| 文章编号： | 1009-9980(2008)03-431-04 |
| 修稿时间： | 2007年11月20 |
Construction of a plant expression vector and transient gene expression in Kumquat(Fortunella crassifolia) |
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| ZHANG Lan-lan,LIU Xiao-hua,CHEN Kun-song,XU Chang-jie.Construction of a plant expression vector and transient gene expression in Kumquat(Fortunella crassifolia)[J].Journal of Fruit Science,2008,25(3):431-434. |
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| Authors: | ZHANG Lan-lan LIU Xiao-hua CHEN Kun-song XU Chang-jie |
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| Abstract: | A plant expression vector, pLZ13, was constructed by replacing the GUS gene in pBI121 with the intron-GUS (iGUS) gene from pCambia1301. GUS staining of Agrobacterium cells containing pCambia1301 or pLZ13 indicated that GUS staining was prevented in both cases. A transient expression study was carried out with kumquat tissues as samples, and no difference was observed for the GUS expression patterns driven by the CaMV 35S promoters from the newly constructed vector pLZ13 and pCambia1301. The vector pLZ13 was a plant expression vector, both suitable for transient gene expression and transgenic research. |
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| Keywords: | Kumquat (Fortunella crassifolia) iGUS Transient expression Promoter Transgenic Plant expression vector |
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