水稻野败型细胞质雄性不育恢复基因的ISSR和SSLP标记分析

以野败型细胞雄性不育系珍油９７Ａ与其强恢复系ＩＲ２４配组,构建由２１０株组成的Ｆ２代极端不育群体作为恢复基因定位群体。用ＩＳＳＲ引物ＵＢＣ８３５对两个亲本进行扩增,发现ＰＣＲ指纹在两个亲本间具多态性,再用此引物对基因定位群体进行连锁分析表明,ＵＢＣ８３５与水稻野败型细胞质雄性不育恢复基因紧密连涣,交换率为３．５７％,转换成图距为３．５８ｃＭ。经过１５０多对微卫星引物进行扫描,在第１、第７染色体和第

Analysis of the Fertilily Restorer Gene in the Wild-abortive (WA) Type Cytoplasmic Male Sterility (CMS) System with the ISSR and SSLP Markers

To develop the PCR based molecular marker closely linked to the restorer gene in the wild abortive (WA) type cytoplasmic male sterility (CMS) system, the F 2 excessive sterile population consisting of 210 plants from the cross between Zhenshan 97A and the strong restorer line IR24 was used for mapping. A ISSR marker,UBC 835, was found to generate polymorphism between the two parents. Linkage analysis in the mapping population showed that UBC 835 was closely linked to the restorer gene of the CMS WA system. The exchange frequency was 3.57%,the distance between UBC 835 and the restorer gene was 3.58 cM. The same results was obtained by linkage analysis with OSR33 located on chromosome 10 in the rice genome by screening more than 150 SSR loci,however, no SSR locus was found to link with the restorer gene in the polymorphic SSR loci on the chromorsome 1 and 7. The use of the two PCR based molecular marker closely linked to the restorer gene in molecular assistant selection for restorer line was also discussed.