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| 植物基因克隆技术的发展与展望 | |
| 引用本文： | 陈儒钢,巩振辉,逯明辉,李大伟,黄炜. 植物基因克隆技术的发展与展望[J]. 长江蔬菜, 2009, 0(20). DOI: 10.3865/j.issn.1001-3547.2009.20.004 |
| 作者姓名： | 陈儒钢 巩振辉 逯明辉 李大伟 黄炜 |
| 作者单位： | 西北农林科技大学园艺学院,陕西杨凌,712100 |
| 基金项目： | 国家自然科学基金，"十一五"国家科技支撑计划，西北农林科技大学"青年学术骨干支持计划"，西北农林科技大学人才基金 |
| 摘 要： | 抑制性差减杂交技术和基因芯片技术是近年来发展起来的研究基因差异表达的2种非常有效的方法.SSH技术或基因芯片技术单独使用都存在不同程度的缺陷和不足,但若将2种技术结合起来使用,则能充分发挥各自的优势,并最大限度地弥补各自的不足.鉴于此,对SSH技术与基因芯片技术的原理与优缺点以及两者的结合在植物研究上的应用进行了综述. |
| 关 键 词： | 抑制性差减杂交(SSH) 基因芯片 差异表达基因 应用 |
Development and Prospect of the Gene Cloning Technology in Plant |
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| CHEN Rugang,GONG Zhenhui,LU Minghui,LI Dawei,HUANG Wei. Development and Prospect of the Gene Cloning Technology in Plant[J]. Journal of Changjiang Vegetables, 2009, 0(20). DOI: 10.3865/j.issn.1001-3547.2009.20.004 | |
| Authors: | CHEN Rugang GONG Zhenhui LU Minghui LI Dawei HUANG Wei |
| Affiliation: | CHEN Rugang,GONG Zhenhui,LU Minghui,LI Dawei,HUANG Wei ( College of Horticulture,Northwest Agriculture , Forest University,Yangling,Shanxi,712100 ) |
| Abstract: | Suppression subtractive hybridization (SSH) and gene chip are two effective methods developed in recent years, which are used to screen all differential expression genes. However, SSH and gene chip have certain limitation respectively. It will be a perfect clone system when they are coupled with each other to exert their advantages and to make up their disadvantages. So in this article, the fundamentals, advantages and disadvantages of SSH and gene chip, the technique characteristic and the application of S... |
| Keywords: | Suppression subtractive hybridization (SSH) Gene chip Differential expression genes Application |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |