| 氧化乐果降解菌株发酵培养基碳氮源优化的研究 |
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| 引用本文： | 王立东,阮长青,郎双静.氧化乐果降解菌株发酵培养基碳氮源优化的研究[J].农产品加工．学刊,2010(1):33-36,52. |
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| 作者姓名： | 王立东 阮长青 郎双静 |
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| 作者单位： | 1. 黑龙江省农产品加工工程技术研究中心,黑龙江,大庆,163319
2. 黑龙江八一农垦大学食品学院,黑龙江,大庆,163319 |
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| 摘 要： | 为确定氧化乐果降解菌株L-3发酵培养基碳源和氮源的最优配比,在质量浓度为1.0mg/mL的氧化乐果降解菌株的基础发酵培养基中,添加不同的碳源和氮源,选出适合菌株生长的最佳碳源和氮源,采用正交试验设计,优化出碳氮源的最佳配比,并进一步测定菌株L-3的生长情况和对氧化乐果的降解效果。结果表明,菌株L-3发酵培养基中最佳的碳源和氮源分别为蔗糖和NH4NO3;最佳的发酵条件为A3B2C3碳源质量浓度0.6g/L,氮源质量浓度0.5g/L,pH值7.0;最适的碳氮质量比为6∶5;菌株L-3在第3天达到生长高峰期,第4天达到降解高峰期,其降解氧化乐果的能力高达86%。优化后的菌种培养基更利于菌体生长,提高了氧化乐果的降解效果。
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| 关 键 词： | 氧化乐果 菌株L-3 发酵 培养基 优化 |
Study on Optimizing the Carbon and Nitrogen Source of Fermention Medium of Folimat Degradation Strain |
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| Wang Lidong,Ruan Changqing,Lang Shuangjing.Study on Optimizing the Carbon and Nitrogen Source of Fermention Medium of Folimat Degradation Strain[J].Nongchanpin Jlagong.Xuekan,2010(1):33-36,52. |
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| Authors: | Wang Lidong Ruan Changqing Lang Shuangjing |
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| Institution: | 1. Foodstuff College/a>;Heilongjiang August First Land Reclamation University/a>;Daqing/a>;Heilongjiang 163319/a>;China/a>;2. Agri-food Processing Development Center of Heilongjiang/a>;China |
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| Abstract: | For ascertaining the optimization matching of C/N of folimat L-3 strains in the fermentation medium,it needed to add to different carbon source(glucose\cane sugar\lactose\fructose\amidulin) and different nitrogen source((NH4) 2SO4\ NH4NO3\urea\folima) t at foundation fermention medium with the 1.0 mg/mL concentration of folimat,by which,the best carbon source and nitrogen source can be found out. One step further,the best matching of carbon source and nitrogen source were optimized by adopting orthogonal ex... |
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| Keywords: | folimat L-3 strains fermentation medium optimization |
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