Determination of Ploidy Level and Nuclear DNA Content in Tunisian Populations of Atriplex halimus L. |
| |
| Authors: | Kheiria Hcini David J. Walker Sadok Bouzid Elena González Nora Frayssinet Enrique Correal |
| |
| Affiliation: | (1) Faculté des Sciences de Tunis, Département des Sciences Biologiques, Laboratoire de Botanique et Biologie Végétale, 2092 El-Manar II Tunis, Tunisia;(2) Departamento de Recursos Naturales y Desarrollo Rural, Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), C/Mayor, s/n La Alberca, 30150 Murcia, Spain;(3) Facultad de Ciencias Exactas y Naturales, UNPSJB – Comodoro Rivadavia, Argentina;(4) Facultad de Agronomía, Universidad de Buenos Aires, Avenida San Martín 4453, Buenos Aires, Argentina |
| |
| Abstract: | Atriplex halimus L. (Chenopodiaceae) (Saltbush) is a perennial species used as a fodder shrub for livestock in arid and semi-arid areas, particularly in North Africa. The aim of this work was to determine whether differences in ploidy level and/or nuclear DNA content exist among populations from widely-separated sites in Tunisia. We determined nuclear DNA content and chromosome numbers for populations of A. halimus from seven different locations (Gabes, Medenine, Tataouine, Monastir, Tunis, Sidi Bouzid, Kairouan). The chromosome counts showed that all the Tunisian populations, plus a population from Eraclea (Italy), were tetraploid (2n = 4x = 36) whereas a population from Cala Tarida (Spain) was diploid (2n = 2x = 18). With respect to nuclear DNA, the 2C DNA content of population Cala Tarida was estimated to be 2.41 pg. There was no significant difference among the tetraploid populations (or among plants within populations), whose 2C DNA content ranged from 4.92 to 4.97 pg. |
| |
| Keywords: | Atriplex halimus L. Flow cytometry Nuclear DNA content Ploidy |
| 本文献已被 SpringerLink 等数据库收录！ |
|
