不同类型纤维对活体外瘤胃发酵和共轭亚油酸形成的影响

研究不同类型的纤维添加向日葵油对活体外瘤胃发酵和共轭亚油酸(CLA)形成的影响。以等量的玉米秸细胞壁、甜菜渣细胞壁和微晶纤维素作为底物,分别代表木质化纤维、易消化纤维和纯纤维素,添加相同水平(占底物DM的4.76%)的向日葵油,用混合瘤胃微生物进行活体外发酵培养24 h。结果表明,添加向日葵油进行活体外发酵培养时,不同纤维类型显著影响活体外瘤胃发酵特性(P<0.01),并影响发酵液脂肪酸组成和CLA的比例。木质化纤维显著地增加(P<0.05)活体外瘤胃微生物对cis9,trans11-CLA的合成。

Effect of different types of fiber on rumen fermentation and production of conjugated linoleic acids in vitro

An in vitro study was conducted to determine the effect of different types of fiber supplemented with sunflower oil on rumen fermentation and formation of conjugated linoleic acids(CLA) by mixed ruminal microorganisms.Cell wall components extracted from corn stover and beet pulp,and avicel were used as substrates,representing lignified fiber,easily digestible fiber and purified cellulose,respectively.Sunflower oil was supplemented at the same level(4.76% of substrate DM) for all three types of fiber.After 24?h of incubation,rumen fermentation parameters and the concentration of fatty acids(FA) in the culture fluid were determined.Results showed that the type of fiber significantly influenced(P<0.01) rumen fermentation traits in vitro.Composition of FA and profile of CLA were altered by the fiber type.Compared to the digestible fiber and purified cellulose,lignified fiber significantly increased(P<0.05) the production of cis9,trans11-CLA by rumen microorganisms.