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| 用~(60)Coγ射线诱导水稻抗瘟突变体的筛选 | |
| 引用本文： | 臧威,张淑园,张国民,李柱刚,孙剑秋,张兰兰,严善春.用~(60)Coγ射线诱导水稻抗瘟突变体的筛选[J].核农学报,2008,22(3):248-253. |
| 作者姓名： | 臧威 张淑园 张国民 李柱刚 孙剑秋 张兰兰 严善春 |
| 作者单位： | 1. 齐齐哈尔大学生命科学与工程学院,黑龙江齐齐哈尔,161006;东北林业大学林学院,黑龙江,哈尔滨,150040 2. 齐齐哈尔大学生命科学与工程学院,黑龙江齐齐哈尔,161006 3. 黑龙江省农业科学院,黑龙江,哈尔滨,150086 4. 东北林业大学林学院,黑龙江,哈尔滨,150040 |
| 基金项目： | 农业部引进国际先进农业科学技术计划("948"计划) |
| 摘 要： | 以水稻为材料,通过60Coγ射线辐射诱变,结合组织培养技术,采用以黑龙江省优势稻瘟病菌谱系产生的混合粗毒素提取液作为选择压力,进行继代培养和分化培养筛选抗瘟水稻突变体。研究表明,水稻成熟胚经过60Coγ射线辐照后,获得的愈伤组织诱导率很低而且褐化率很高,但是通过苗瘟抗性鉴定发现,再生植株中对稻瘟病表现抗性的植株比例较大,说明辐射诱发突变与生物技术相结合的抗病育种途径具有较大的应用潜力和较好的发展前景。 |
| 关 键 词： | 稻瘟病 粗毒素 组织培养 辐射诱变育种 抗瘟突变体 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2009-12-31 |
SCREENING OF RESISTANT MUTANT INDUCED BY γ-RAYS IRRADIATION TO RICE BLAST |
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| ZANG Wei,ZHANG Shu-yuan,ZHANG Guo-min,LI Zhu-gang,SUN Jian-qiu,ZHANG Lan-lan,YAN Shan-chun.SCREENING OF RESISTANT MUTANT INDUCED BY γ-RAYS IRRADIATION TO RICE BLAST[J].Acta Agriculturae Nucleatae Sinica,2008,22(3):248-253. | |
| Authors: | ZANG Wei ZHANG Shu-yuan ZHANG Guo-min LI Zhu-gang SUN Jian-qiu ZHANG Lan-lan YAN Shan-chun |
| Abstract: | Rice seeds were irradiated with ~(60)Co γ-rays to induce mutants,which resistant to rice blast were screened in subculture medium and differentiation medium under mix toxin from dominant strains of Pyricularia grisea in Heilongjiang.The results indicated that the inductivity of callus of mature rice embryo by irradiation was very low and the browning rate of callus was very high.However,the resistance rate of resistant regenerating plants was obviously higher,which indicated the resistant breeding by irradiation and biotechnology had a great potential and a better prospect. |
| Keywords: | rice blast phytotoxin tissue culture mutation breeding by irradiation resistant mutant |
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