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| 通过双重PCR方法区分坏死梭杆菌亚种 | |
| 引用本文： | 姚志利,刘晓颖,陈立志,冯凯,王燕,刘振铸,李玉文.通过双重PCR方法区分坏死梭杆菌亚种[J].特产研究,2010,32(1):15-18. |
| 作者姓名： | 姚志利 刘晓颖 陈立志 冯凯 王燕 刘振铸 李玉文 |
| 作者单位： | 1. 中国农业科学院特产研究所,吉林,吉林,132109 2. 长春市双阳区农业综合执法大队,吉林,长春,130600 3. 唐山市汉洁管理区动物卫生监督所,河北,唐山,301501 |
| 基金项目： | "十五"国家科技攻关子课题，吉林省科技发展计划项目，吉林市科技发展计划项目 |
| 摘 要： | 基于已发表的坏死梭杆菌白细胞毒素启动子区序列,设计3条特异性引物L7、L8和L9。L7和L8分别与Fnn和Fnf的特异性序列结合,L9与两个亚种序列相匹配。分别扩增出1 076bp和809bp的特异性片段。试验结果表明,这些引物具有高度的特异性和敏感性,能够检测坏死梭杆菌基因组DNA的最小量为10pg/μL,建立的双重PCR体系可用于区分坏死梭杆菌亚种。 |
| 关 键 词： | 坏死梭杆菌 双重PCR 白细胞毒素 |
Differentiation of Fusobacterium necrophorum Subspecies by Duplex PCR |
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| YAO Zhi-li,LIU Xiao-ying,CHEN Li-zhi,FENG Kai,WANG Yan,LIU Zhen-zhu,LI Yu-wen.Differentiation of Fusobacterium necrophorum Subspecies by Duplex PCR[J].Special Wild Economic Animal and Plant Research,2010,32(1):15-18. | |
| Authors: | YAO Zhi-li LIU Xiao-ying CHEN Li-zhi FENG Kai WANG Yan LIU Zhen-zhu LI Yu-wen |
| Institution: | YAO Zhi-li1,LIU Xiao-ying1,CHEN Li-zhi1,FENG Kai2,WANG Yan1,LIU Zhen-zhu2,LI Yu-wen3(1.Institute of Special wild Economic Animals , Plants,CAAS,Jilin 132109,China,2.Agricultural Synthetic Group in Shuangyang District of Changchun City,Changchun 130600,3.Animal Health Supervision in Hongu Administrative District of Tangshan City,Tangshan 301501,China) |
| Abstract: | Thee pairs of specific： primers （L7, L8 and L9 ）were designed based on the published leukntoxin gene sequences of Fusobacterium necrophorum. L7 and Lg targeting Fnn and Fnf, respectively;L9 targeting both subspecies. Fnn and Fnf amplify 1 076 bp and 809 bp fragments respectively. Experimental results showed that these primers possessed a high specificity and sensitivity,we can detect genome DNA 20 pg/Q74 L. These results sugguest that duplex PCR can be used as a new tool for the differentiation of Fusobacterium necrophorum subspecies. |
| Keywords: | Fusobacterium necrophorum duplex PCR leukotoxin |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |