| 影响农杆菌介导的河北杨遗传转化的因素 |
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| 引用本文： | 贾小明,樊军锋.影响农杆菌介导的河北杨遗传转化的因素[J].西北林学院学报,2006,21(5):102-105. |
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| 作者姓名： | 贾小明 樊军锋 |
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| 作者单位： | 西北农林科技大学,林学院,陕西,杨陵,712100 |
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| 基金项目： | 国家转基因植物与产业化专项基金;西北农林科技大学校科研和教改项目;国家林业局重点实验室基金 |
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| 摘 要： | 从预培养时间、侵染菌液浓度、侵染时间、共培养时间及添加A S(乙酰丁香酮)5个方面,以卡那霉素抗性芽频率作为衡量指标,研究了各因素对河北杨遗传转化率的影响。优化筛选的最适转化系统为:预培养2 d,农杆菌活化菌液1/2M S稀释10倍,侵染5 m in,共培养4 d。按此方法河北杨叶盘转化率可达35%。
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| 关 键 词： | 河北杨 遗传转化 转化率 |
| 文章编号： | 1001-7461（2006）05-0102-04 |
| 收稿时间： | 2005-12-06 |
| 修稿时间： | 2005-12-26 |
Factors Affecting Agrobacterium Mediated Transformation of Populus hopeiensis |
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| JIA Xiao-ming,FAN Jun-feng.Factors Affecting Agrobacterium Mediated Transformation of Populus hopeiensis[J].Journal of Northwest Forestry University,2006,21(5):102-105. |
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| Authors: | JIA Xiao-ming FAN Jun-feng |
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| Abstract: | The effects of influencing factors were studied,such as pre-culture time,Agrobacterium concentration,infection time,co-culture time and adding AS on genetic transformation frequency of Populus hopeiensis,of which the frequency of kanamycin resistance buds was used as measure standard.The superior transformation system was as following,pre-cultured two days and then infected 5 minutes in Agrobacterium diluted ten times with 1/2MS liquid medium,co-cultured four days at last.The transformation frequency of leaf disc of Populus hopeiensis would reach to 35% with this system. |
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| Keywords: | Populus hopeiensis genetic transformation transformation frequency |
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