苹果自花授粉的花粉管结构变化及S-RNase定位

 苹果‘红星’品种授粉处理24 h后, 自花授粉比异花授粉的花粉管生长缓慢。授粉处理48 h后, 可观察到自花授粉花柱通道组织的花粉管内有二裂粒子增加、管壁破裂和管内物质溢出、外层管壁增厚等现象。苹果气球状花期未授粉的花柱及自花和异花授粉24 h后且无花粉管存在的花柱切片显示, S-RNase位于花柱通道组织的细胞内。自花和异花授粉48 h后有花粉管通过的花柱切片显示, S-RNase位于花柱通道组织的细胞间隙和花粉管内。

Observation on Structural Changes of Self-pollinated Pollen Tube and Localization of S-RNase in Apple

Structural changes in the pollen tubes of the ‘Starking Delicious’apple in vivo was investigated by transmission electron microscope. Itwas found that a slow growth of the pollen tube after 24 h for the self-pollination. Compared with the cross-pollination, there were numerous bipartite particles in the pollen tube cell and some changes in 48 h for the self-pollination, either appeared such as rupture of the pollen tube, release of the cellularmaterials, thickening of the outer walls of some pollen tube cells. At the time without pollination, or 24 h after self- and cross-pollination, in the samp les that pollen tubes did not exist, the S-RNase was localized in the stylar transmitting tissue cells. However, in 48 h after pollination with self- or cross-pollen, in the samp les that pollen tubes existed, the S-RNase was observated in the intercellular space or into pollen tubes.