Extreme resistance to infection by potato virus X in genotypes of wild tuber-bearingSolanum species |
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| Authors: | Alejandro Carlos Tozzini María Fernanda Ceriani María Verónica Saladrigas H. Esteban Hopp |
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| Affiliation: | (1) Instituto de Biología Molecular CICV-INTA Castelar, CC 77, 1708 Morón, Argentina;(2) Catedra de Genética, Facultad de Agronomia, Universidad de Buenos Aires, Avenida San Martin 4453, 1417 Buenos Aires, Argentina |
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| Abstract: | Summary Clones derived from thirty-one different accessions (nineteen of Argentine origin) belonging to eightSolanum species were screened for resistance to infection by potato virus X strain cp (PVX cp) by mechanical inoculation of plantlets that had been micropropagated in vitro. Estimates of PVX multiplication obtained by enzyme linked immunosorbent assay and slot blot nucleic acid hybridization allowed the identification of resistant clones derived from five accessions belonging toS. commersonii S. oplocense, S. sparsipilum andS. tuberosum andigena. Resistant genotypes supported PVX concentrations 5 to 15 times smaller than did the susceptible control cultivar Spunta. Graft inoculation test confirmed the presence of extreme resistance similar to that conferred by the ‘immunity’ gene X1 (also called RXact). |
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| Keywords: | Solanum commersonii Solanum gourlayi Solanum incamayoense Solanum neorossii Solanum oplocense Solanum sparsipilum Solanum tuberosum sppandigena Solanum vidaurrei |
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