Cryopreservation of Embryogenic Callus Cultures from Barley (Hordeum vulgare L.) |
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| Authors: | G. Hahne, H. Lö rz |
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| Affiliation: | Dr. G. Hahne;, IBMP du CNRS, 12, Rue du Général Zimmer, 67084 Strasbourg Cedex (France) Dr. H. Lörz;, Max-Planck-Institut für Züchtungsforschung, Egelspfad, D-5000 Köln 30 (German, F.R.). |
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| Abstract: | ![]()
Embryogenic callus cultures of barley (Hordeum vulgare L.) were frozen in the presence of a cryoprotector and subsequentjy stored in liquid nitrogen. After thawing, a high percentage of cultures resumed growth after a lag-period of 2–4 weeks. Plant regeneration was achieved at a frequency comparable to that observed in control cultures. |
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| Keywords: | Hordeum vulgare barley cryopreservation embryogenic callus somatic embryos |
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