甜叶菊叶片灭菌方法的研究

为筛选出适宜甜叶菊叶片灭菌的条件,以甜叶菊叶片为外植体,通过统计污染率、枯死率和愈伤组织诱导率来评价灭菌效果,研究不同灭菌剂、灭菌时间对灭菌效果的影响。结果表明:单一灭菌剂的灭菌效果不好,以0.1%HgCl2灭菌5min的愈伤组织诱导率较高,为44.5%;75%酒精与其它灭菌剂组合的灭菌效果好于单一灭菌剂,以75%酒精与0.1%HgCl2组合最好,污染率和枯死率较低,愈伤组织诱导率较高,适宜甜叶菊叶片灭菌的组合为75%酒精4s+0.1%HgCl24min,愈伤组织诱导率达86.7%。

Study on the Sterilization Method of Stevia rebaudiana Leaves

In order to screen appropriate sterilization conditions of Stavia rebaudiana leaves, taking the leaves of Stevia rebaudiana as explants, by statisticsing the contamination rate, exsciccation rate and induction rate of callus to appraise the sterilization effect,the sterilization effects of different sterilants and different sterilization time on explants were studied. The results showed that sterilization effect of single sterilizing agent was not satisfactory,the higher induction rate of callus was only 44.5 % with H gCl2 at the concentration of 0. 1%;the sterilization effect of alcohol and other sterilizing agent combination were better than that of single sterilizing a- gent, the best combination was alcohol （75 % ） and HgCl2 （ 0. 1%） with the lowest pollution rate, withered rate and the highest induction rate of callus, the suitable sterilizing time and combination for Stevia rebaudiana leaves was alcohol（75 % ）for four second with HgCl2 （0.1% ）for four minute,and the induction rate of callus a- chieved 86.7%.