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水稻条纹病毒楚雄分离物一个重组RNA序列分析
引用本文:程文金,吴祖建,谢联辉.水稻条纹病毒楚雄分离物一个重组RNA序列分析[J].中国农学通报,2009,25(18):352-355.
作者姓名:程文金  吴祖建  谢联辉
作者单位:福建农林大学植物病毒研究所,福建省植物病毒学重点实验室,福州,350002
基金项目:农业部公益性行业(农业)科研专,国家自然科学基金项目 
摘    要:测序鉴定水稻条纹病毒(Rice stripe virus, RSV)云南楚雄分离物(YCX07)的重组片段YCX07R。该片段由RSV RNA2的3'端序列与RNA3的3'端序列重组而成,具有RSV基因组结构特征,采取双义编码策略,即在正、负链的5'端分别存在一个阅读框,编码两个重组蛋白YCX07R-5P、YCX07R-3P。推测重组系由RNA流产性复制引起或重组的两个片段通过重组点直接联结形成。

关 键 词:RSV  重组RNA  双义编码
收稿时间:2009-04-20
修稿时间:2009-05-11

A recombinant RNA from Chuxiong isolate of Rice stripe virus(RSV) was sequenced
Cheng Wenjin,Wu Zujian,Xie Lianhui.A recombinant RNA from Chuxiong isolate of Rice stripe virus(RSV) was sequenced[J].Chinese Agricultural Science Bulletin,2009,25(18):352-355.
Authors:Cheng Wenjin  Wu Zujian  Xie Lianhui
Institution:(Key Lab of Plant Virology of Fujian Province, Institute of Plant Virology,Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002)
Abstract:A recombinant RNA of one isolate of Rice stripe virus(RSV) from Chuxiong(YCX07), Yunnan Province, China, was sequenced. The RNA was recombined from the 3' segments of RNA2 and RNA3 of RSV. The recombinant RNA uses ambisense coding strategy and encodes two proteins, namely YCX07R-5P and YCX07R-3P. The two proteins located on the 5' end of the plus and minus strand, respectively. This is similar to the four RNAs of RSV. It was discussed that the RNA was recombined from abortion replication or linked by two recombinant fragments through the recombinant site directly.
Keywords:RSV
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