小肽与酵母培养物对舍饲牦牛瘤胃微生物多样性和发酵参数的影响

为研究添加小肽与酵母培养物对舍饲育肥牦牛瘤胃微生物多样性和发酵参数的影响,试验选取36头年龄为4周岁左右、体重水平相当、健康状况良好的麦洼牦牛,随机分为4个处理组,对照组饲喂全混合日粮(TMR),试验Ⅰ、Ⅱ、Ⅲ组在TMR基础上分别添加1％酵母培养物、0.75％小肽、1％酵母培养物+0.75％小肽.试验结果表明:(1)四...

Effects of small peptide and yeast culture on rumen microbial diversity and fermentation parameters of yaks

The aim of this study was to investigate the effects of adding small peptides and yeast cultures on the apparent digestibility,production performance and blood biochemical parameters of the fattening calves.Thirty-six wheat yak aged 4 years old,with similar body weight and good health were randomly divided into 4 treatment groups,control group(TMR full mixed diet),experiment group I(TMR+1%yeast culture),experiment group II(TMR+0.75%small peptide),experiment group III(TMR+1%yeast culture+0.75%small peptide).The results showed that:(1)The dominant bacteria in the four treatment groups were Bacteroidetes,Firmicutes,Synergistests,Proteobacteria.Small peptide and yeast culture significantly reduced the relative abundance of Proteobacteria(P<0.05),the relative abundance of Bacteroides and Synergistests increased,but the difference was not significant(P>0.05).Subordinate level analysis,Bacteroidetes BS11 gut group,Rikenellaceae RC9 gut group,Prevotella 1,Christensenellaceae R 7 group for four treatment groups has a dominant genus.Addition of small peptides and yeast cultures significantly increased the relative abundance of the Rikenellaceae RC9 gut group,Prevotellaceae_UCG-004,Ruminococcaceae_UCG-010 and Papillibacter,Mollicutes_RF9(P<0.05).(2)The addition of small peptide and yeast culture alone or at the same time had no significant effect on pH and ammonia nitrogen concentration in the rumen(P>0.05);the concentration of acetic acid in the rumen was decreased,but the difference was not significant(P>0.05);The 1%yeast culture group and 1%yeast culture+0.75%small peptide group significantly increased the concentration of propionic acid in the rumen(P<0.05),while the0.75%small peptide group increased the concentration of propionic acid in the rumen,but the difference was not significant(P>0.05);small peptide and yeast culture alone or simultaneously added significantly reduced the acetic acid/propionic acid value(P<0.05),indicated that the rumen fermentation from acetic acid type to propionic acid type conversion,which was conducive to the improvement of energy use efficiency.In summary,the addition of small peptides and yeast cultures alone or simultaneously promote the growth of beneficial rumen bacteria,transform rumen fermentation from acetic acid to propionic acid,and improve energy utilization efficiency,small peptides and yeast cultures have additive effect.