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| 家蚕来源肠球菌RAPD反应体系的优化 | |
| 引用本文： | 宋连花,王彦文,牟志美,黄艳红. 家蚕来源肠球菌RAPD反应体系的优化[J]. 河北林果研究, 2006, 21(1): 78-81 |
| 作者姓名： | 宋连花 王彦文 牟志美 黄艳红 |
| 作者单位： | 1. 山东农业大学,林学院,山东,泰安,271018 2. 山东农业大学,林学院,山东,泰安,271018;四川农业大学,动物科技学院,四川,雅安,625014 |
| 基金项目： | 国家茧丝绸发展风险基金 |
| 摘 要： | 通过调整 Mg2 、DNA模板、引物、Taq DNA聚合酶和dNTPs的浓度及PCR反应循环次数,建立并优化了家蚕来源肠球菌的RAPD-PCR反应体系及条件.结果表明,在25 μl反应体系下,当Mg2 的浓度为3.5 mmol/L、DNA模板加入量为50 ng、引物浓度为15 pmol/L、Taq DNA聚合酶加入量为3U、 PCR反应循环次数为45时,扩增结果最好.在本实验室,为家蚕来源肠球菌的遗传多样性研究建立了一种最佳RAPD分析模型. |
| 关 键 词： | 家蚕 肠球菌 随机扩增DNA多态性分析 条件优化 |
| 文章编号： | 1007-4961(2006)01-0078-04 |
| 修稿时间： | 2005-11-08 |
Optimization on the conditions of RAPD analysis in enterococci isolated from Bombyx mori |
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| SONG Lian-hua,WANG Yan-wen,MU Zhi-mei,HUANG Yan-hong. Optimization on the conditions of RAPD analysis in enterococci isolated from Bombyx mori[J]. Hebei Journal of Forestry and Orchard Research, 2006, 21(1): 78-81 | |
| Authors: | SONG Lian-hua WANG Yan-wen MU Zhi-mei HUANG Yan-hong |
| Abstract: | The influences of concentration of Mg~(2 ),DNA template,primer,T(aq) polymerase,dNTPs and number of cycles had been tested for the amplification of random amplified polymorphic DNA(RAPD) in Enterococci of Bombyx mori. The results showed that when Mg~(2 ) at 3.5 mmol/L,DNA template at 50 ng,primer at 15 pmol/L,T(aq) polymerase at 3U,dNTPs at 200 mmol/L and the number of cycles was 45,the amplification performed perfectly.An optimization RAPD analysis pattern for the genetic research in Enterococci of Bombyx mori was established. |
| Keywords: | Bombyx mori Enterococci random amplified polymorphic DNA(RAPD) conditions optimization |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |