Genetic diagnosis of Rehmannia species micropropagated by tip tissue culture and an F1 hybrid by RAPD analysis

Shoot formation was induced from the tip tissue of Rehmannia glutinosa f. hueichingensis on hormone-free Murashige-Skoog medium within 4 weeks of culture. Shoots were propagated on Murashige-Skoog medium supplemented with l.0mg/l benzy0ladenine for 4 weeks. Propagated shoots rooted on the hormone-free Murashige-Skoog medium during 4 weeks of culture. Total DNA was extracted from the leaves of a F₁ hybrid and its parents. R. glulinosa f. huekliingensis and R. glutinosa var, purpurea. Analysis of random-amplified polymorphic DNA (RAPD) using 10 arbitrary oligonucleotide 10-mers. showed the genetic homogeneity ofthe above three species. The F₁ hybrid was genetically intermediate between both parental plants, compared with the genetic distance between the F₁ hybrid and individual parents. Furthermore, the comparison of the band patterns between the F₁ hybrid, obtained from the crossing clearly showed that parts of the bands of both parents. R. glutinosa f. hueichingensis and R. glutinosa var. purpurea, were introduced into the F₁ hybrid.