| 烟草CMV抗性鉴定及抗性基因的SSR标记研究 |
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| 引用本文： | 范静苑,王元英,蒋彩虹,陈万胜,任民,胡海洲.烟草CMV抗性鉴定及抗性基因的SSR标记研究[J].分子植物育种,2009,7(2). |
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| 作者姓名： | 范静苑 王元英 蒋彩虹 陈万胜 任民 胡海洲 |
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| 作者单位： | 中国农业科学院烟草研究所,青岛,266101 |
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| 基金项目： | 国家烟草专卖局科技项目，院所基金基本科研业务费专项基金 |
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| 摘 要： | 本研究以抗黄瓜花叶病毒病的烟草品种铁把子和台烟8号,感病品种NC82和中烟15为亲本,构建F1、F2、BC1代分离群体,并对它们进行CMV的接种鉴定和抗性遗传分析,结果表明铁把子和台烟8号对CMV的抗性是由1对隐性基因控制的.依据混合群体分组分析法(BSA),从F2代群体植株中选取DNA建立黄瓜花叶病毒病的抗病基因池和感病基因池,利用135对SSR引物进行分子标记和分析,得到标记SM1350与来源于铁把子的CMV抗性基因间的遗传距离为8.64 cM,标记SM2270与来源于台烟8号的CMV抗性基因间的遗传距离为3.92 cM.
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| 关 键 词： | 烟草 黄瓜花叶病毒 抗性遗传分析 |
Resistance Identification and SSR Marking of Resistance Gene of Tobacco Cucumber Mosaic Virus |
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| Fan Jingyuan,Wang Yuanying,Jiang Caihong,Chen Wansheng,Ren Min,Hu Haizhou.Resistance Identification and SSR Marking of Resistance Gene of Tobacco Cucumber Mosaic Virus[J].Molecular Plant Breeding,2009,7(2). |
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| Authors: | Fan Jingyuan Wang Yuanying Jiang Caihong Chen Wansheng Ren Min Hu Haizhou |
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| Institution: | Fan Jingyuan Wang Yuanying Jiang Caihong Chen Wansheng Ren Min Hu Haizhou Tobacco Research Institute of China Academy of Agricultural Sciences,Qingdao,266101 |
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| Abstract: | The study was carried out using F1, F2, BC1 populations from a cross between CMV resistant vari- eties tiebazi/taiyan-8 and CMV susceptible varieties NC82/zhongyan15. They were identified by artificial inoc- ulation on seedlings in greenhouse and the inheritance of resistance were analyzed. Results showed that the re-sistant character of both resistant parents is controlled by single recessive gene. DNAs of F2 population were extracted to develop into a resistant pool and a sensitive pool based on the BSA (... |
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| Keywords: | SSR |
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