采用双峰驼重链抗体特异的抗血清检测不同抗原免疫骆驼过程中血清重链抗体的水平

为了探究重链抗体在骆驼免疫保护中的生物学作用,本研究利用Protein G和Protein A亲和层析纯化新疆双峰驼血清中总IgG和重链抗体IgG2,并免疫昆明小白鼠制备抗双峰驼总IgG和抗双峰驼重链抗体IgG2的多抗血清;通过ELISA检测双峰驼在体液免疫应答过程中针对spaA-N、溶菌酶和蒜氨酸酶3种抗原的重链抗体的滴度变化。结果从新疆双峰驼血清中亲和层析纯化出天然重链抗体IgG2,蛋白质分子质量约为46 ku;免疫昆明小白鼠后获得抗双峰驼总IgG多抗血清的效价为1∶204800,抗双峰驼重链抗体IgG2多抗血清的效价为1∶6400。在spaA-N免疫骆驼诱导的体液免疫应答过程中,重链抗体IgG2出现延迟反应。3种蛋白均能诱导抗原特异的IgG2亚类重链抗体的产生。

The Dynamic Change of Heavey Chain Antibody in Camelus bactrianu during the Immunization with Different Antigens Assayed by Heavy Chain Antibody Specific Antiserum

In order to explore heavy chain antibodies(Cb-HcAb) biological effects of immune protection in Xinjiang Camelus bactrianus.Purification of IgG subclass in camel serum by protein G and protein A affinity chromatography,and preparation of anti-camel total IgG and IgG2 antiserum by immunization of Kunming white mice.The level of spaA-N,lysozyme and alliinase specific IgG2 and total IgG in camel serum during the immunization were analyzed with ELISA.Purification of IgG2 subclass from Xinjiang Camelus bactrianus serum by affinity chromatography with molecular weight of 46 ku.Mice anti-camel total IgG serum titer and anti-IgG2 serum titer were 1∶204800 and 1∶6400 respectively.During the immunization in camel with different antigens,all antigen specific-IgG2 HcAbs were induced during the immunization in camel,in which anti-spaA-N specific IgG2 HcAb occurred delay in the process of camel humoral immune response.