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| 一株苯酚降解菌的筛选、鉴定及其降解特性 | |
| 引用本文： | 刘鸿杰,何熙璞,张敏,梁锦添,陈加辉,李俊芳,陈保善.一株苯酚降解菌的筛选、鉴定及其降解特性[J].广西农业生物科学,2011,30(3):336-341. |
| 作者姓名： | 刘鸿杰 何熙璞 张敏 梁锦添 陈加辉 李俊芳 陈保善 |
| 作者单位： | 1. 广西大学化学化工学院,南宁,530004 2. 广西大学生命科学与技术学院,南宁,530004 3. 亚热带农业生物资源保护与利用国家重点实验室,南宁,530004 |
| 基金项目： | 广西大学基金项目，广西环境工程与保护评价重点实验室开放课题，广西研究生教育创新计划资助项目 |
| 摘 要： | 本研究采用逐量分批驯化的方法,从造纸废水中分离得到一株能够以苯酚为唯一碳源生长的苯酚降解菌株F5-1。经形态观察、生理生化特性鉴定及16S rDNA序列分析,将该菌株鉴定为克雷伯菌（Klebsiella sp.）。该菌株能够在7h时完全降解初始浓度为100mg/L的苯酚,降解苯酚主要发生在生长对数期;在pH5.0～9.0,NaCl浓度0～80g/L,温度20～40℃范围内,菌株F5-1均可有效降解初始浓度为100～1200mg/L的苯酚;能够耐受的最大苯酚浓度为1500mg/L。本研究结果表明,F5-1菌株对处理环境条件复杂的含酚废水具有潜在的应用前景。 |
| 关 键 词： | 苯酚 16S rDNA 生物降解 克雷伯菌 分离鉴定 |
Screening,Identification and Degrading Characteristics of A Phenol Degrading Bacterial Strain |
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| Liu Hongjie,He Xipu,＊ Zhang Min,Liang Jintian,Chen Jiahui,Li Junfang,Chen Baoshan.Screening,Identification and Degrading Characteristics of A Phenol Degrading Bacterial Strain[J].Journal of Guangxi Agricultural and Biological Science,2011,30(3):336-341. | |
| Authors: | Liu Hongjie He Xipu ＊ Zhang Min Liang Jintian Chen Jiahui Li Junfang Chen Baoshan |
| Institution: | 2,3＊ 1 College of Chemistry and Chemical Engineering,Guangxi University,Nanning,530004;2 Guangxi Key Laboratory of Subtropical Bio-resource Conservation and Utilization,Nanning,530004;3 College of Life Science and Technology,Guangxi University,Nanning,530004 |
| Abstract: | By gradually increasing the concentration of phenol in the culture medium,a phenol-degrading bacterial strain designated as F5-1 was isolated from the wastewater of a paper mill.The organism was able to utilize phenol as a sole carbon source.Based on morp |
| Keywords: | Phenol 16S rDNA Biodegradation Klebsiella sp Isolation and identification |
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