| Scriptaid处理可提高近交系五指山猪克隆胚胎的发育能力和克隆效率 |
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| 引用本文： | 朱彦宾,龙川,冯冲,潘登科,罗玉柱.Scriptaid处理可提高近交系五指山猪克隆胚胎的发育能力和克隆效率[J].广西农业生物科学,2011,30(3):268-273. |
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| 作者姓名： | 朱彦宾 龙川 冯冲 潘登科 罗玉柱 |
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| 作者单位： | 1. 甘肃农业大学甘肃省草食动物生物技术重点实验室／动物科学技术学院,兰州,730070
2. 中国农业科学院北京畜牧兽医研究所,北京,100193 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金，国家重大专项 |
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| 摘 要： | 为探讨一种新型低毒的组蛋白去乙酰化酶抑制剂Scriptaid处理克隆胚胎时对其发育能力和克隆效率的影响,本研究以近交系五指山小型猪胎儿成纤维细胞为供体细胞进行体细胞核移植构建重构胚胎,重构胚胎激活后培养在添加Scriptaid不同浓度（0～300nmol/L）的胚胎培养液中培养不同的时间（0～36h）,观察克隆胚胎的卵裂率和囊胚率,评价克隆胚胎体外的发育能力。实验结果发现100nmol/L Scriptaid处理24h组克隆胚胎的囊胚发育率（30.4%）较对照组（17.5%）显著提高,P〈0.05。将100nmol/L Scriptaid处理24h组克隆胚胎和对照组胚胎分别移植到4头受体母猪中,进一步观察其体内的发育能力。处理组克隆胚胎的受体在平均窝产仔数和克隆效率（分别为5头,2.4%）均显著高于对照组（分别为1.5头,0.7%）,P〈0.05。以上结果表明,100nmol/L Scriptaid处理24h近交系五指山小型猪克隆胚胎,有利于提高克隆胚胎的发育能力和克隆效率。
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| 关 键 词： | 近交系小型猪 Scriptaid 核移植胚胎 克隆效率 |
Improving the Developmental Potency of Cloned Embryos and Cloning Efficiency in Wuzhishan Inbred Pig by Scriptaid Treatm |
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| Zhu Yanbin,Long Chuan,Feng Chong,Pan Dengke,＊ Luo Yuzhu.Improving the Developmental Potency of Cloned Embryos and Cloning Efficiency in Wuzhishan Inbred Pig by Scriptaid Treatm[J].Journal of Guangxi Agricultural and Biological Science,2011,30(3):268-273. |
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| Authors: | Zhu Yanbin Long Chuan Feng Chong Pan Dengke ＊ Luo Yuzhu |
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| Institution: | 1＊ 1 Gansu Key Laboratory of Herbivores Animal Biotechnology/Faculty of Animal Sci-Tech,Gansu Agricultural University,Lanzhou,730070;2 Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing,100193 |
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| Abstract: | The present study was designed to examine the effect of Scriptaid,a low toxicity deacetylase inhibitor,on developmental potency of cloned embryos and cloning efficiency.The cloned embryos were reconstructed with Wuzhishan inbred miniature pig fetal fibrob |
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| Keywords: | Inbred miniature pig Scriptaid Nuclear transfer embryo Cloning efficiency |
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