| 相对定量法分析小鼠CRBN基因表达 |
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| 引用本文： | 张俭斌,陈佩林.相对定量法分析小鼠CRBN基因表达[J].安徽农业科学,2010(25):13628-13630. |
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| 作者姓名： | 张俭斌 陈佩林 |
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| 作者单位： | 南通大学生命科学学院,江苏南通,226007;南通大学生命科学学院,江苏南通,226007 |
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| 摘 要： | 目的]研究CRBN基因与常染色体隐性遗传非综合征轻型精神发育迟滞（ARNSMR）的关系,探讨小鼠CRBN基因mRNA在不同组织中的表达情况。方法]应用RT-PCR和Real-time PCR技术,研究了小鼠CRBN基因在肺、肝、心脏、肾和大脑中的表达情况。结果]小鼠CRBN基因mRNA在肺、大脑中表达量较高,在心脏中表达量最低。结论]小鼠CRBN基因广泛表达于不同组织,除了与学习、记忆功能相关外,可能还有其他功能。
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| 关 键 词： | CRBN RT-PCR Real-time PCR |
mRNA Expression profile of CRBN Gene in Different Mouse Tissues |
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| ZHANG Jian-bin et al.mRNA Expression profile of CRBN Gene in Different Mouse Tissues[J].Journal of Anhui Agricultural Sciences,2010(25):13628-13630. |
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| Authors: | ZHANG Jian-bin |
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| Institution: | ZHANG Jian-bin et al (School of Life Sciences,Nantong University,Nantong,Jiangsu 226007) |
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| Abstract: | Objective]In order to explore the relationship of CRBN gene with autosomal recessive nonsyndromic mental retardation (ARNSMR),we analyze mRNA expression profile of CRBN gene in different mouse tissues.Methods]By using RT-PCR and Real-time PCR,we examine expression of mouse CRBN gene in lung,liver,heart,kidney and brain.Result]Result shows that CRBN is expressed at an extremely high level in mouse lung and brain,while at an extremely low level in heart.Conclusion]Mouse CRBN gene is expressed widely.It ma... |
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| Keywords: | CRBN RT-PCR Real-time PCR |
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