| 原生质体电融合再生柑橘属间四倍体体细胞杂种 |
| |
| 引用本文： | 刘继红,邓秀新. 原生质体电融合再生柑橘属间四倍体体细胞杂种[J]. 中国农业科学, 2000, 33(2): 98-100 |
| |
| 作者姓名： | 刘继红 邓秀新 |
| |
| 作者单位： | 华中农业大学作物遗传改良国家重点实验室,武汉,430070 |
| |
| 基金项目： | 国家自然科学基金!资助项目 ( 3 962 50 1 7) |
| |
| 摘 要： | 自 Ohgawara等 (1 985)得到枳与 Trovita甜橙属间体细胞杂种植株近 1 5年来 ,已得到了约 1 50例柑橘体细胞杂种〔3〕,其中有种、属间有性和嫁接亲和的组合 ,也有有性和嫁接均不亲和的属间、族间组合。这些体细胞杂种的获得 ,丰富了柑橘的种质资源库 ,为育种提供了可供选择和利用的新型试材。本文报道的是最近获得的一例金柑与柑橘属间体细胞杂种。1 材料与方法1 .1 材料丹西红橘 (Citrusreticulata )愈伤组织悬浮系继代 3次后 ,于第 7～ 1 0天时取愈伤组织分离原生质体 ;长寿金柑 (Fortunella obovata )种子播种于 MT基本培养基 (含 3 …
|
| 关 键 词： | 金柑 柑橘 原生质体 电融合 体细胞杂种 |
Production of Tetraploid Intergeneric Somatic Hybrid Plants Via Protoplast Electrofusion in Citrus |
| |
| Liu Jihong,Deng Xiuxin. Production of Tetraploid Intergeneric Somatic Hybrid Plants Via Protoplast Electrofusion in Citrus[J]. Scientia Agricultura Sinica, 2000, 33(2): 98-100 |
| |
| Authors: | Liu Jihong Deng Xiuxin |
| |
| Abstract: | Mesophyll protoplasts of Changshou kumquat (Fortunella obovata) were fused electrically with embryogenic protoplasts of Dancy tangerine (Citrus reticulata). Plants were obtained after culture of the fusion treated protoplasts. Cytological examinations showed that the plants were tetraploids with 36 chromosomes. RAPD analysis indicated that the plants had bands specific to the fusion partners in the primers (OPA 04 and OPA 07) chosen for verifying hybridity of the plants. The results demonstrated that tetraploid intergeneric somatic hybrid plants have been recovered by fusion of protoplasts between kumquat and citrus. |
| |
| Keywords: | Fortunella obovata Citrus Protoplast Electrofusion Somatic hybrids |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农业科学》浏览原始摘要信息 |
|
点击此处可从《中国农业科学》下载全文 |
