| 转基因烟草的耐盐性研究 |
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| 引用本文： | 黄绍兴,刘俊君,阎隆飞,彭学贤.转基因烟草的耐盐性研究[J].中国农业大学学报,1995(Z1):1-7. |
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| 作者姓名： | 黄绍兴 刘俊君 阎隆飞 彭学贤 |
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| 作者单位： | 北京农业大学生物学院;中国科学院微生物研究所;中国科学院微生物研究所 北京; |
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| 摘 要： | 通过植物基因工程技术,获得了分别表达编码细菌1-磷酸甘露醇脱氢酶基因(mtlD)和6-磷酸山梨醇脱氢酶基因(gutD)的转基因烟草。Southern和Northernblot结果表明,mtlD基因和gutD基因均已整合到烟草染色体DNA中,并转录产生对应的mRNA。糖醇含量测定表明,在所测试的mtlD基因转化烟草中,均有不同剂量的甘露醇的合成;在gutD基因转化烟草中,gutD的表达导致了细胞内山梨醇相对浓度的提高。在含1.75%NaClHoagland溶液中的耐盐实验表明,转化植株的耐盐性比非转化植株的大为增强。
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| 关 键 词： | 大肠杆菌 mtlD 基因 gutD 基因 转基因烟草 糖醇合成 耐盐性 |
Studies on Salt Tolerance of Transgenic Tobacco |
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| Huang Shaoxing Yen Longfei College of Biology,BAU.Studies on Salt Tolerance of Transgenic Tobacco[J].Journal of China Agricultural University,1995(Z1):1-7. |
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| Authors: | Huang Shaoxing Yen Longfei College of Biology BAU |
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| Institution: | Huang Shaoxing Yen Longfei College of Biology,BAU.Beijing 100094,Liu Junjun Peng Xuexian Institute of Microbiology,Chinese Academy of Sciences,Beijing 100080 |
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| Abstract: | Transgenic tobacco plants which expressed bacterial mannitol-1-phosphate dehydrogenase gene(mtlD) and glucitol-6-phosphate dehydrogenase gene (gutD) respectively were obtained by plant ge- netic engineering.The results of Southern and Northern blot analysis confirmed that mtID gene had inte- grated into the genomic DNA of tobacco plants and produced specific mRNAs.Tests of sugar alcohols indi- cated that different content of mannitol had been produced in mtlD transgenic tobacco plants,and the ex- pression o... |
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| Keywords: | Escherichia coli mtlD gene gutD gene transgenic tobacco plant production of sugar alcohols salt tolerance |
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