| 黄瓜花原基启动细胞团及其组织化学的研究 |
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| 引用本文： | 陶月良,曾广文,朱诚.黄瓜花原基启动细胞团及其组织化学的研究[J].浙江大学学报(农业与生命科学版),2001,27(2):134-138. |
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| 作者姓名： | 陶月良 曾广文 朱诚 |
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| 作者单位： | 1. 温州师范学院生物与环境科学系，浙江温州 325003
2. 浙江大学生物科学系，浙江杭州 310029 |
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| 基金项目： | 浙江省自然科学基金资助项目（396061） |
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| 摘 要： | 以黄瓜幼苗为材料，通过花芽分化形态学、组织切片及显微图像定量仪对细胞内DNA,RNA和蛋白质含量的测定等研究，确定了花原基的启动细胞团及其形成计时，并对花原基启动细胞团进行了组织化学研究，结果表明，花原基启动细胞团的细胞内DNA,RNA和蛋白质的含量以及RNA/DNA的含量明显高于未启动时叶腋表皮下的细胞，说明花原基启动分化期间进行着活跃的生理代谢活动。Ca2+定位研究表明，叶腋表皮下细胞内的Ca2+颗粒少，主要分布于液泡。而花原基启动细胞团细胞的胞壁和细胞间隙中分布着大量的Ca2+颗粒。
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| 关 键 词： | 黄瓜 花原基 启动 组织化学 |
| 文章编号： | 1008-9209(2001)02-0134-05 |
The Studies on the
formation and timing of evocation cell group of floral primordia and its histochemistry in
Cucumber |
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| TAO Yue liang ,ZENG Guang wen ,ZHU Chen.The Studies on the
formation and timing of evocation cell group of floral primordia and its histochemistry in
Cucumber[J].Journal of Zhejiang University(Agriculture & Life Sciences),2001,27(2):134-138. |
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| Authors: | TAO Yue liang ZENG Guang wen ZHU Chen |
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| Institution: | TAO Yue liang 1,ZENG Guang wen 2,ZHU Chen 2 |
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| Abstract: | The present paper investigated the formation and its timing of evocation cell group of floral primordia by morphogenesis of floral differentiation, tisssue section, and its histochemistry in cucumber. The results showed that the evocation cell group of floral primordia occurred in subepiderm at the first axils of the seedlings of 6 day age. The content of DNA, RNA and Protein, and the ratio of RNA/DNA are higher in the cell of evocation cell group than in the cells of non evocation. Moreover a large amount of calcium ion occurred in the evocation cells(cell wall and intercellular spaces). |
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| Keywords: | Cucumis sativus floral primordium evocation histochemistry |
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