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| 烟蚜茧蜂畸形细胞对寄主生理代谢的调控 | |
| 引用本文： | 刘金文 颜秀娟,丛 斌. 烟蚜茧蜂畸形细胞对寄主生理代谢的调控[J]. 中国农学通报, 2011, 27(9): 368-371 |
| 作者姓名： | 刘金文 颜秀娟 丛 斌 |
| 作者单位： | 1. 吉林省农业科学院党委办公室,长春,130033 2. 吉林省农业科学院大豆研究中心,长春,130033 3. 沈阳农业大学植物保护学院,沈阳,110161 |
| 摘 要： | 【研究目的】研究畸形细胞对寄主蚜虫的生理生化的影响及其自身的发展变化。【方法】分别采用Folin-酚法、SDS聚丙烯酰胺凝胶电泳法、薄层层析法和索氏抽提法测定寄生和未寄生寄主血淋巴的蛋白质含量、蛋白质组成、糖类含量和脂肪含量变化。【结果】寄生寄主与未寄生寄主相比，生理代谢受到抑制。血淋巴蛋白和甘油酯含量随日龄逐渐增加到第3天达最大后下降，而糖含量逐渐降低，分析是畸形细胞分泌和合成的结果。同时，经SDS-PAGE电泳显示，血淋巴中新出现15.9kDa和51.1kDa两种特异的蛋白质。【结论】通过对寄主血淋巴主要成分的分析，烟蚜茧蜂畸形细胞对寄主的发育起着重要的调控作用。 |
| 关 键 词： | 调查 调查 |
| 收稿时间： | 2010-09-28 |
| 修稿时间： | 2010-11-26 |
Effects of Aphidius gifuensis teratocytes on physiological metabolism of aphis |
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| Liu Jinwen,Yan Xiujuan,Cong Bin. Effects of Aphidius gifuensis teratocytes on physiological metabolism of aphis[J]. Chinese Agricultural Science Bulletin, 2011, 27(9): 368-371 | |
| Authors: | Liu Jinwen Yan Xiujuan Cong Bin |
| Affiliation: | Liu Jinwen 1,Yan Xiujuan 2,Cong Bin 3(1 Secretary of Party Committee,Academy of Agricultural Sciences of Jilin Province,Changchun 130033,2 Soybean Research Centre,3 College of Plant Protection,Shenyang Agricultural University,Shenyang 110161) |
| Abstract: | Investigate effects of Aphidius gifuensis teratocytes on physiological metabolism of aphis and change themself.In the study,folin-phenol,SDS-PAGE,thin layer chromatography and soxhlet extractor method were used to determine the content of protein,protein composition,the quantity of sugar and glyceride for haemolymphe in parasitized and non-parasitized host.It was indicated that the metabolism of parasitized aphid was constantly inhibited as compared with that of the non-parasitized host.The level of protein... |
| Keywords: | Aphidius gifuensis teratocytes physiological metabolism regulate aphid |
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