| 利用RAMP-PCR技术对枸杞10个品种资源的分析 |
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| 引用本文： | 梁海永,刘兴菊,杨敏生.利用RAMP-PCR技术对枸杞10个品种资源的分析[J].中国农学通报,2011,27(16):61-64. |
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| 作者姓名： | 梁海永 刘兴菊 杨敏生 |
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| 作者单位： | 河北农业大学林学院,河北保定,071000 |
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| 基金项目： | 国家林业局行业公益项目 |
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| 摘 要： | 以枸杞为材料，建立基因组DNA的有效提取方法，并对10个枸杞品种进行了RAMP技术分析研究。结果表明，利用10对引物共扩增出87个条带，其中多态性条带53个；多态性条带比率33.3%~81.9%，10个枸杞品种间的遗传距离为0.1111~0.8333；研究表明，利用RAMP进行枸杞的品种DNA指纹分析是可行的。
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| 关 键 词： | 种植业结构 种植业结构 现状 评价 聊城市 |
| 收稿时间： | 2011/1/26 0:00:00 |
| 修稿时间： | 3/2/2011 12:00:00 AM |
Analysis of Lycium chinense Variety Revealed by RAMP-PCR Method |
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| Liang Haiyong,Liu Xingju,Yang Minsheng.Analysis of Lycium chinense Variety Revealed by RAMP-PCR Method[J].Chinese Agricultural Science Bulletin,2011,27(16):61-64. |
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| Authors: | Liang Haiyong Liu Xingju Yang Minsheng |
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| Institution: | Liang Haiyong,Liu Xingju,Yang Minsheng ( Forestry College,Agricultural University of Hebei,Baoding Hebei 071000) |
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| Abstract: | To develop valid methods of DNA extraction for Lycium chinense for RAMP analysis and research. The result showed that: ten primers were applied to amplification. A total of 87 DNA bands were detected. 53 bands among which were polymorphic. The rate of polymorphic bands was 33.3%-81.9%. 10 Lycium chinense cultivars genetic distances were from 0.1111 to 0.8333. In the study, the RAMP markers can be used for the studies of fingerprint of Lycium chinense sensitively. |
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| Keywords: | Lycium chinense RAMP marker genomic DNA germplasm |
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