小麦重组自交系成株抗条锈性QTL分析

 【目的】对小麦成株期条锈病抗性进行数量性状位点（QTL）分析。【方法】以小麦重组自交系内乡188/偃展1号为材料,在连续两年田间充分发病的情况下,分别用病程曲线下面积（Area Under Disease Progress Curve,AUDPC）和反应型（Infection Type,IT）2种病情指标,通过复合区间作图,分析成株抗条锈性的加性QTL、上位性互作及其分别与环境的互作效应（QTL×environment interaction,QE）。【结果】两年共检测到9个加性抗性QTL,其中使用AUDPC和IT共检测到2个相同的QTL;9个QTL中,5个具有环境互作效应。还检测到7对上位性互作的QTL,其中2对具有环境互作效应。采用AUDPC数据,检测到的QTL能够解释表型的62.05%,其中主要为加性效应（44.32%）和上位性互作效应（17.73%）,环境互作很小（0.42%）。采用IT数据,总共检测到的QTL解释了表型变异的37.53%,其中加性效应和上位性互作效应分别解释了23.94%和10.51%,与环境互作解释了3.08%。【结论】内乡188的抗条锈性是由多个位点控制的,在感病亲本偃展1号中也存在抗性QTL;位于3B和6D染色体上的QTL为2个新的成株期抗性条锈位点;非抗性位点间存在上位性互作效应。

Quantitative Trait Loci for Adult-Plant Resistance Against Yellow Rust in a Wheat-Derived Recombinant Inbred Line Population

Objective] This study aimed at detecting quantitative trait loci (QTL) responsible for adult resistance against wheat yellow rust.Method] In this study,a recombinant inbred line (RIL) population derived from a cross between Neixiang 188 and Yanzhan 1 was evaluated for adult-plant resistance (APR) in two years under field conditions using AUDPC (Area Under Disease Progress Curve,AUDPC) and IT (Infection Type,IT).Result] Nine additive QTLs and seven pairs of epistatic QTLs were detected based on the composite interval mapping.Among them,five additive QTLs and two pairs of epistatic QTLs showed QTL×environment interactions (QEs).Two QTLs,QYr.caas-3D and QYr.caas-6A,were both detected using either AUDPC or IT data.The total QTL effects based on AUDPC data explained 62.05% phenotype variation,with 44.32% additive effects,17.73% epistatic effects,and 0.42% QEs.Using IT data,the total QTLs explained 37.53%,with 23.94% additive effects,10.51% epistatic effects,and 3.08% QEs.Conclusion] The loci of adult plant resistance to yellow rust were identified not only in resistant parent Neixiang 188,but also in susceptible parent Yanzhan 1.QYr.caas-3B and QYr.caas-6D were two new loci responsible for the resistance to yellow rust disease.The interaction could occur between two loci without resistaace.