拟南芥Nodulin MtN21家族At1g0938基因功能的初步研究

拟南芥Nodulin MtN21 蛋白家族含有1～2个DUF6结构域，具有内在膜蛋白的特性。推测该家族基因在小分子跨膜运输、信号转导和抗逆性等方面发挥作用。At1g09380是拟南芥Nodulin MtN21 基因家族成员。本研究通过PCR筛选获得At1g09380对应的T-DNA 插入纯合子，并通过RT-PCR鉴定该突变体为无效突变体。对突变体萌发和萌发后阶段进行盐胁迫和干旱胁迫分析，结果表明，At1 g09380 突变体萌发后对NaCl和干旱胁迫敏感，证明该基因参与拟南芥的盐和干旱胁迫响应过程。同时还发现在长日照条件下突变体植株比野生型植株早开花7d左右。以上研究结果为进一步分析该基因的生物学功能打下基础。

Primary Analysis of the Function of Nodulin MtN21 Gene At1 g09380 in Arabidopsis thaliana

Nodulin MtN21 protein family contains one to two DUF6 domain signature characteristics of integral membrane proteins.The protein family is predicted to play very important roles in transport of micromolecule transmembrane,signal transduction and stress-resistance.At1 g09380 is a member of Nodulin MtN21 gene family.One homozygous T-DNA mutant was identified by PCR and RT-PCR as null mutant.The phenotype of the mutants which were observed at the stages of germination and postgermination showed that At1 g09380...