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| 微载体培养技术及其在疫苗生产中的应用 | |
| 引用本文： | 王家敏,平玲,徐水林,马桂兰,魏园园,乔自林,马忠仁,令世鑫.微载体培养技术及其在疫苗生产中的应用[J].黑龙江农业科学,2014(12):146-149. |
| 作者姓名： | 王家敏 平玲 徐水林 马桂兰 魏园园 乔自林 马忠仁 令世鑫 |
| 作者单位： | 1. 甘肃省动物细胞工程技术研究中心,甘肃兰州,730030 2. 兰州民海生物工程有限公司,甘肃兰州,730010 3. 兰州百灵生物技术有限公司,甘肃兰州,730010 |
| 基金项目： | 教育部长江学者和创新团队发展计划资助项目(IRT13091);甘肃省科技计划资助项目(1203FKDA030、2013GS08044);兰州市科技计划资助项目(2013-4-101) |
| 摘 要： | 微载体培养技术是一种新兴的细胞大规模培养技术,是目前贴壁依赖型细胞大规模培养的重要方法,其兼具贴壁培养和悬浮培养的优势,温度、溶氧浓度和pH等培养条件易控制,培养过程自动化、系统化,不易被污染。现就微载体培养技术的发展及其在疫苗生产中的应用进行了综述,并提出了动物细胞微载体大规模培养技术的发展方向。 |
| 关 键 词： | 微载体 细胞培养 疫苗 |
Microcarrier Culture Technique and Its Application in Vaccine Production |
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| Institution: | WANG Jia-min , PING Ling , XU Shui-lin , MA Gui-lan , WEI Yuan-yuan , QIAO Zi-lin , MA Zhong-ren (1. Gansu Engineering Research Center for Animal Cell, Lanzhou, Gansu 730030 ; 2. Lanzhou National Hyclone Bio-Engineering Company Limited, Lanzhou, Gansu 730010; 3. Lanzhou Lark Bio-Tech Company Limited, Lanzhou, Gansu 730010) |
| Abstract: | Microcarrier cell culture technique is a new cell culture technology,which is primary method of largescale culture of adherent-dependent cell currently.It possesses both adherent and suspension culture advantages,which is easy to control culture conditions such as temperature,oxygen concentration,pH and so on,and its culturing process is systematic,automated and hard to be contaminated.The development and application in vaccine production of microcarrier culture technology were summarized,and the development of large-scale animal cell microcarrier culture technique was proposed. |
| Keywords: | microcarrier cell culture vaccine |
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