A rapid and simple method for determination of potato chloroplast DNA type |
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Authors: | Kazuyoshi Hosaka Robert E. Hanneman |
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Affiliation: | 1. Department of Horticulture, University of Wisconsin, 53706, Madison, WI 2. USDA, Agricultural Research Service, Department of Horticulture, University of Wisconsin, 53706, Madison, WI
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Abstract: | A procedure for restriction enzyme analysis of the potato chloroplast DNA (ctDNA) is described. The advantages of this method are: 1) rapid determination of ctDNA type, 2) no ultracentrifugation, and 3) low cost of analyses. This method makes it easy to distinguish the ctDNA types of wild and cultivated potato accessions. |
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