| 小麦抗叶锈病基因Lr35的RGA分析 |
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| 引用本文： | 高倩,王海燕,刘大群,李星,杨文香.小麦抗叶锈病基因Lr35的RGA分析[J].华北农学报,2008,23(6). |
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| 作者姓名： | 高倩 王海燕 刘大群 李星 杨文香 |
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| 作者单位： | 河北农业大学,植物病理系,河北省农作物病虫害生物防治工程技术研究中心,河北,保定,071001 |
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| 基金项目： | 河北省教育厅资助课题(Z2007408); 河北省自然科学基金(C2008000281) |
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| 摘 要： | 根据已知植物抗病基因的NBS，LRR等保守结构域设计引物，对小麦全套抗叶锈病近等基因系材料进行RGA分析。引物对Pto-kin1IN／XLRR-INV1从近等基因系TeLr35中扩增获得一条747bp的特异性片段。序列分析表明，该片段与小麦基因片段Triticum urartu clone BAC 210J24，Triticum monococcum DV92的BAC克隆231A16等具有较高同源性，为Lr35的克隆奠定了基础。
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| 关 键 词： | 小麦叶锈病 抗病基因 Lr35 抗病基因类似序列 |
RGA Analysis of the Wheat Leaf Rust Resistance Gene Lr35 |
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| GAO Qian,WANG Hai-yan,LIU Da-qun,LI Xing,YANG Wen-xiang.RGA Analysis of the Wheat Leaf Rust Resistance Gene Lr35[J].Acta Agriculturae Boreali-Sinica,2008,23(6). |
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| Authors: | GAO Qian WANG Hai-yan LIU Da-qun LI Xing YANG Wen-xiang |
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| Abstract: | Based on the conserved domains(NBS,LRR etc)of the known resistance genes,primers were designed to amplify resistant homologous sequences in the near isogenic lines(NILs)of wheat leaf rust resistance using RGA method.A 747 bp specific fragment was found in TcLr35 by primer pair Pto-kin1IN/XLRR-INV1.The specific fragment was isolated from the polyacrylamide gels,re-amplified,cloned and sequenced.The result indicated that it has the high homology with the Triticum urartu clone BAC 210J24,Triticum monococcum DV... |
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| Keywords: | Wheat leaf rust disease Resistance gene Lr35 Reistance gene analogys |
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