超声波辅助提取菜用黄麻色素及其抗氧化活性的研究

采用单因素试验分析和正交试验设计,研究超声波辅助提取菜用黄麻色素的工艺条件,用Fenton法测定菜用黄麻色素的抗氧化活性.结果表明:(1)影响色素提取的因素为料液比>超声频率>提取温度>提取时间;(2)提取的最佳条件为使用60∶40(V/V)的0.1%盐酸和95%乙醇作为提取剂,料液比1∶80(g.mL-1),超声频率为中频,提取时间30 min,提取温度为60℃;以此工艺条件提取色素的产率达25.04%;(3)该色素溶液还原力强,具有清除超氧阴离子自由基O2.-和.OH的活性,在色素浓度为0.20 g.L-1时,对超氧阴离子自由基O2.-的清除率为70.11%,对.OH的清除率为83%.

Extraction of the pigment from jute with ultrasonic assisted method and its antioxidant activity

Extraction technology of pigment from jute with ultrasonic assisted method was determined with single-factor and orthogonal experimental design.Antioxidant activity of extracted pigment was evaluated with Fenton method.The factors affecting extraction rate were ordered as ratios of raw material to solvent >ultrasonic frequency>extraction temperature>extraction time.The optimum conditions were 60∶ 40(V/V) of 0.1% HCl to 95% alcohol,1∶ 80 of raw material to solvent,intermediate ultrasonic frequency,extraction time 30 min,extraction temperature 60 ℃.Under these conditions,yield of jute pigment was 25.04%.The pigment had reducing activity and scavenging capacity on O-·2 and ·OH.Scavenging rate on O-·2 was 70.11%,that on ·OH was 83% when the concentration was 0.20 g·L-1.