饲料中添加生物素对草鱼幼鱼生长、体成分和血清生化指标的影响

在纯化饲料中分别添加生物素0、0.05、0.10、0.20、0.40、0.80、1.60 mg/kg投喂初始质量为(5.92±0.25)g的草鱼(Ctenopharyngodon idellus)幼鱼8周,研究了不同生物素添加量对草鱼幼鱼生长性能、饲料系数、机体营养成分、血清生化指标的影响。试验结果显示:与对照组相比,添加生物素提高了草鱼幼鱼的增重率、特定生长率,降低了饲料系数。添加量为0.40 mg/kg时草鱼幼鱼的特定生长率和增重率最大,饲料系数最低,并与对照组存在显著差异(P<0.05);添加不同水平生物素对草鱼幼鱼全鱼水分、粗蛋白、粗脂肪含量无显著影响,但添加量为0.40 mg/kg时粗蛋白含量最大。0.10 mg/kg组和0.20 mg/kg组的全鱼灰分含量显著高于对照组(P<0.05);添加生物素对血清总蛋白(TP)、血糖(GLU)和总胆固醇(TC)无显著影响,但显著提高了血清甘油三酯(TG)含量,各添加组TG含量均显著高于对照组(P<0.05),1.60 mg/kg添加组的高密度脂蛋白胆固醇(HDL-C)和低密度脂蛋白胆固醇(LDL-C)含量显著高于对照组(P<0.05)。综合本试验结果,草鱼幼鱼饲料中生物素适宜添加量为0.40 mg/kg。

Effects of dietary biotin on growth,body composition and serum biochemical indexes of juvenile grass carp(Ctenopharyngodon idellus)

An 8-week feeding trial was conducted to investigate growth,feed efficiency,body composition,serum biochemical indexes of juvenile grass carp(Ctenopharyngodon idellus).Purified diets with 7 levels(0,0.05,0.10,0.20,0.40,0.80,1.60 mg/kg) of supplemental biotin were fed to juvenile grass carp with mean weight of(5.92±0.25)g for 8 weeks.Each diet was fed to three replicate groups of fish.All fish were fed at a rate of 2%~3% of their body weight per day in three equal feedings.The results showed that:The addition of biotin to diets improved specific growth rate(SGR),weight gain rate(WGR) of fish,while declined feed conversion rate(FCR).Statistical evaluation showed significant differences in the 0.40 mg/kg group compared to control group(P<0.05).The highest SGR and WGR were observed at the 0.40 mg/kg group,while FCR were lowest.Moisture,crude lipid and crude protein contents of body were not markedly affected by the supplemental levels of dietary biotin but the crude protein content of 0.40 mg/kg group was the highest in all groups,the ash content of 0.10 mg/kg group and 0.20 mg/kg group were significantly higher than control group(P<0.05).Biotin supplementation had no significant influence on total protein(TP),glucose(GLU),total cholesterol(TC) to grass carp with.The total triglyceride(TG) of fish in experimental groups was significantly higher than control group(P<0.05).The low density lipoprotein cholesterol(LDL-C) and high density lipoprotein cholesterol(HDL-C) were significantly higher than control group when the fish fed 1.60 mg biotin per kg diet(P<0.05).Results indicated that the supplementation of biotin to diets for juvenile grass carp was 0.40 mg/kg.